Fine structure of E. coli RNA polymerase-promoter interactions: α subunit binding to the UP element minor groove
Abstract
The α subunit of E. coli RNAP plays an important role in the recognition of many promoters by binding to the A+T-rich UP element, a DNA sequence located upstream of the recognition elements for the ς subunit, the −35 and −10 hexamers. We examined DNA–RNAP interactions using high resolution interference and protection footprinting methods and using the minor groove-binding drug distamycin. Our results suggest that α interacts with bases in the DNA minor groove and with the DNA backbone along the minor groove, but that UP element major groove surfaces do not make a significant contribution to α binding. On the basis of these and previous results, we propose a model in which α contacts UP element DNA through amino acid residues located in a pair of helix–hairpin–helix motifs. Furthermore, our experiments extend existing information about recognition of the core promoter by ς70 by identifying functional groups in the major grooves of the −35 and −10 hexamers in which modifications interfere with RNAP binding. These studies greatly improve the resolution of our picture of the promoter–RNAP interaction.
Keywords
Footnotes
-
↵3 Present address: Medicinal Chemistry, Preclinical Drug Research, Schering AG, D-13342 Berlin, Germany.
-
↵4 Corresponding author.
-
E-MAIL rgourse{at}bact.wisc.edu; FAX (608) 262 9865.
-
Article and publication are at www.genesdev.org/cgi/doi/10.1101/gad.870001.
-
- Received November 29, 2000.
- Accepted January 16, 2001.
- Cold Spring Harbor Laboratory Press