Self-assembling RNA molecules present compelling substrates for the rational interrogation
and control of living systems. However, imperfect in silico models—even at the secondary …

Therapeutic mRNAs and vaccines are being developed for a broad range of human diseases,
including COVID-19. However, their optimization is hindered by mRNA instability and …

Emerging evidence suggests that the ribosome has a regulatory function in directing how
the genome is translated in time and space. However, how this regulation is encoded in the …

… pre-run at 25 W for 1 h, before loading the 90 C denatured RNA in 70% formamide, 1× TBE
loading buffer with bromophenol blue and xylene cyanol running markers and run at 25 W for …

The discovery and design of biologically important RNA molecules is outpacing three-dimensional
structural characterization. Here, we demonstrate that cryo-electron microscopy can …

RNA-Puzzles is a collective experiment in blind 3D RNA structure prediction. We report
here a third round of RNA-Puzzles. Five puzzles, 4, 8, 12, 13, 14, all structures of riboswitch …

This paper is a report of a second round of RNA-Puzzles, a collective and blind experiment
in three-dimensional (3D) RNA structure prediction. Three puzzles, Puzzles 5, 6, and 10, …

For decades, dimethyl sulfate (DMS) mapping has informed manual modeling of RNA structure
in vitro and in vivo. Here, we incorporate DMS data into automated secondary structure …

Single-nucleotide-resolution chemical mapping for structured RNA is being rapidly advanced
by new chemistries, faster readouts, and coupling to computational algorithms. Recent …

Non-coding RNAs fold into precise base-pairing patterns to carry out critical roles in genetic
regulation and protein synthesis, but determining RNA structure remains difficult. Here, we …