Characterizing the transcriptome of individual cells is fundamental to understanding complex
biological systems. We describe a droplet-based system that enables 3′ mRNA counting …

Understanding mechanisms of late/acquired cancer immunotherapy resistance is critical to
improve outcomes; cellular immunotherapy trials offer a means to probe complex tumor–…

We developed a chemically inducible Cas9 (ciCas9) and a droplet digital PCR assay for
double-strand breaks (DSB-ddPCR) to investigate the kinetics of Cas9-mediated generation …

The accumulation of somatic mitochondrial DNA (mtDNA) mutations contributes to the
pathogenesis of human disease. Currently, mitochondrial mutations are largely considered results …

Despite recent therapeutic progress, advanced melanoma remains lethal for many patients.
The composition of the immune tumor microenvironment (TME) has decisive impacts on …

The traditional diagnostic tests for tuberculosis consist of an acid fast stain and a culture test
from a sputum sample. With the emergence of drug resistant strains of tuberculosis, nucleic …

Recent studies have implicated mitochondrial DNA mutations in tumor progression. In this
chapter, we provide a review of the studies reporting on mitochondrial DNA mutations and …

Despite recent therapeutic progress, advanced melanoma remains lethal for many patients.
The composition of the immune tumor microenvironment (TME) has decisive impacts on …

Mitochondria are critical components of cellular metabolism as mediators of oxidative
phosphorylation. As semi-autonomous organelles, mitochondria possess their own DNA (mtDNA) …

The accumulation of somatic mitochondrial DNA (mtDNA) mutations contributes to the
pathogenesis of human disease. Currently, mitochondrial mutations are largely considered results …