… Burkholder, A. Gragerov, ME Gottesman, and WA Hendrickson are in the Department of …
WF Burkholder and ME Gottesman are also in the Institute of Cancer Research, Columbia …

Chaperones of the Hsp70 family bind to unfolded or partially folded polypeptides to facilitate
many cellular processes. ATP hydrolysis and substrate binding, the two key molecular …

We identified a signaling pathway that prevents initiation of sporulation in Bacillus subtilis
when replication initiation is impaired. We isolated mutations that allow a replication initiation …

Sample heterogeneity often masks DNA methylation signatures in subpopulations of cells.
Here, we present a method to genotype single cells while simultaneously interrogating gene …

Epigenetic alterations are increasingly recognized as causes of human cancers and disease.
These aberrations are likely to arise during genomic reprogramming in mammalian …

Circulating tumour DNA (ctDNA) has the potential to be a specific biomarker for the monitoring
of tumours in patients with colorectal cancer (CRC). Here, our aim was to develop a …

… between Sda and the KinA using Sda to inhibit the Spo0A phosphorelay (Burkholder …
15N/13C-labeled Sda ( 1 mM in and Spo0F-His6) were produced as described (Burkholder et al., …

… Burkholder WF and Grossman AD Regulation of the initiation of endospore formation in
Bacillus subtilis Prokaryotic development. Brun YV and Shimkets LJ 2000 151 -166 American …

… William F Burkholder , William F Burkholder … We thank members of the Goh, Burkholder
and Quake laboratories for feedback and discussions. We also thank J. Ho and E. …

This protocol details a method for measuring the DNA methylation state of multiple target
sites in single cells, otherwise known as single-cell restriction analysis of methylation (SCRAM). …