BACKGROUND Biomolecular mechanisms are typically inferred from static structural “snapshots”
obtained by x-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, …

… the fluorophore into the non-fluorescent state T 1 . The kinetics for switching the fluorophore
… constant k T , that is, the inverse of the triplet-state lifetime. This rate constant k T determines …

… and the acceptor (k T ). E is hence easily determined by comparing the fluorescence
lifetimes τ D of the donor-only species and τ T −1 = k D + k T = τ D −1 + k T for the donor–acceptor …

Immunoresponsive gene 1 (Irg1) is highly expressed in mammalian macrophages during
inflammation, but its biological function has not yet been elucidated. Here, we identify Irg1 as …

Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to
determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, …

Metabolic reprogramming is emerging as a hallmark of the innate immune response, and
the dynamic control of metabolites such as succinate serves to facilitate the execution of …

… advanced signal processing for STED / Gated STED (Giuseppe Vicidomini, Marco Castello
and Alberto Diaspro) and approaches to improve fluorophore photostability (Thorben Cordes)…

Recent advances in photobleaching and blinking prevention have aided the advancement
of single-molecule and super-resolution fluorescence microscopy. However, a common …

Serine, glycine and other nonessential amino acids are critical for tumour progression, and
strategies to limit their availability are emerging as potential therapies for cancer 1 , 2 – 3 . …

… S5A can clearly be assigned to the triplet state with k T in the absence of oxygen being k T
= (5.5 ± 2.5) × 10 3 s −1 (see SI Text for analysis). The independence of the action of AA and …