… Kosuri et al. used predesigned barcodes that allowed PCR amplification of oligos …
Kosuri, S. et al. Scalable gene synthesis by selective amplification of DNA pools from high-…

… Notably these data reveal that the predicted PAM for the S. pyogenes Cas9 is not just NGG
… these experiments was that the predicted PAM for the S. pyogenes Cas9 is not just NGG but …

The ability to direct functional proteins to specific DNA sequences is a long-sought goal in
the study and engineering of biological processes. Transcription activator–like effectors (…

Digital information is accumulating at an astounding rate, straining our ability to store and
archive it. DNA is among the most dense and stable information media known. The …

… In this landmark work, a large library of 2350 poly(β-amino ester)s (PBAEs) were synthesized
by Michael addition of amines to diacrylates. These cationic polymers were prepared in …

Most amino acids are encoded by multiple codons, and codon choice has strong effects on
protein expression. Rare codons are enriched at the N terminus of genes in most organisms, …

The inability to predict heterologous gene expression levels precisely hinders our ability to
engineer biological systems. Using well-characterized regulatory elements offers a potential …

… that reduced IPTG induction, called I s variants 12 , with substitutions near the binding
pocket (Supplementary Table 3). We combined these I s variants with the gentiobiose-responsive …

… Element overlap may also encode uncharacterized function(s) having to do with the regulation
of protein synthesis or the coupling of selective pressures during evolution. For example, …

Abstract Development of cheap, high-throughput and reliable gene synthesis methods will
broadly stimulate progress in biology and biotechnology 1 . Currently, the reliance on column-…