Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome
of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding …

Most small plasmids of Gram-positive bacteria use the rolling-circle mechanism of replication
and several of these have been studied in considerable detail at the DNA level and for the …

… Wild-type cells (no mutations in sip genes) and cells lacking a functional sipS gene were
labelled with [ 35 S]methionine for 60 s prior to chase with non-radioactive methionine. After 5 …

… RNA was isolated by spinning down 2 ml of culture (30 s Eppendorf centrifuge, 14 000 rpm,
4C) and resuspending the pellet in 0.3 ml ice‐cold growth medium (final volume 0.4 ml). The …

… Here, we developed a time-resolved infection model of human lung alveolar epithelial cells
by S… We imaged adherent S. pneumoniae with fluorescence microscopy during the indicated …

… of their gene(s) of interest. The work and data presented here provide a valuable resource
to the pneumococcal and microbial research community and will expand our knowledge of S. …

Bacterial spores are a continuous problem for both food-based and health-related industries.
Decades of scientific research dedicated towards understanding molecular and gene …

… To ensure homogenization of both mother cell and forespore compartments, sporulating
cells were exposed to five 45-s-long bead-beating cycles, with at least 1-min intervals of cooling …

The results of a comparison of three DNA-detection methods for human parvovirus B19
DNA are described. The sensitivity of detection of virus from hybridization assays using 32 P-…

… The Add complex did not appear to affect deletion frequencies in our system (S. Bron,
unpublished results). As shown previously (Peeters et al. 1988) the presence of the IRs raised the …