… For scientists developing miRNA therapeutics, validated targets provide the best biomarker(s)
for determination of the efficacy of a miRNA mimic or inhibitor. The identification of miRNA …

Imagine being able to knock out your favourite gene with only a day's work. Not just in one
model system, but in virtually any organism: plants, flies, mice or cultured cells. This sort of …

Survival for childhood cancer has increased dramatically over the last 40 years with 5-year
survival rates now approaching 80%. For many diagnostic groups, rapid increases in survival …

Zebrafish have become a popular organism for the study of vertebrate gene function 1 , 2 .
The virtually transparent embryos of this species, and the ability to accelerate genetic studies …

… 1A) was loaded on a Mono S column and eluted with a linear KCl gradient. Fractions were
… (B) The fraction with peak activity from the Mono S column in (A) was loaded on a Mono Q …

… We also carried out a screen of 96 miRNAs, to look for miRNA(s) that accelerate Myc-induced …
We thank members of the Hannon, Lowe and Hammond laboratories for discussions and …

RNA interference (RNAi) is the mechanism through which double-stranded RNAs silence
cognate genes 1 , 2 , 3 , 4 , 5 . In plants, this can occur at both the transcriptional and the post-…

… used to target endogenous genes, we transfected S2 cells with a dsRNA corresponding to
the first 540 nucleotides of Drosophila cyclin E, a gene that is essential for progression into S …

Gene silencing through RNA interference (RNAi) is carried out by RISC, the RNA-induced
silencing complex. RISC contains two signature components, small interfering RNAs (siRNAs) …

… Carmell, and members of the Hammond laboratory for critical reading of the manuscript
and discussion of the project. SMH is a General Motors Cancer Research Foundation Scholar. …