… Indeed, much is expected from the next generation(s) of sequencers, which envisage
single-molecule sequencing at an even lower cost and higher speed, and the direct measurement …

… ’s instructions. cDNA was generated using Superscript III (Life Technologies) according to the
manufacturer’s … We thank S. Srivastava for analysing and confirming a SPEM phenotype of …

… An induction of oxidative stress is exemplified by the up-regulation of three peroxidase
precursors and two glutathione S-transferases… Simon Denil is supported by an IWT doctoral grant. …

… Infected and control roots were collected at 7 and 14 dai and fixed in Farmer’s fixative (3:1
ethanol/acetic acid). The material was dehydrated in ethanol and then cryosectioned with a …

… Rapiclear (Sunjin laboratory) was used to clear whole-mount tissues and vibratome sections
according to the manufacturer’s instructions. Hoescht was used as a nuclear counterstain. …

… The PCR conditions were as follows: 95C for 10 min for initial enzyme activation and 40
cycles of amplification at 95C for 15 s and 60C for 1 min. Ct values were exported for web-based …

… modifications: (1) cells were initially seeded into 12-well plates (Corning, 353043) at a
density of 106 cells/well, and (2) stage 1 was extended to 3 days by repeating the final day’s …

… Condition of the MSP was 10 min hot-start at 95 C; 95 C for 60 s, 60 C for 60 s, and 72 C
60 s for a total of 40 cycles, with a final elongation step of 7 min at 72 C. Leukocyte DNA from …

… WM164 parent, IDTC colonies and dabrafenib resistant cells were fixed using 4%
paraformaldehyde for 10 min followed by chromatin preparation according to the manufacturer’s …

… E2 in this article’s Online Repository at www. jacionline. org). … E2 in this article’s Online
Repository at www. jacionline. org). … in this article’s Online Repository at www. jacionline. org). …