… ), 22 water-mediated hydrogen bonds and 41 van der Waal’s (hydrophobic) contacts (<4 Å).
All … coli core is higher than that of σ to Tth core (S Borukhov unpublished data). However, the …

RNA polymerase (RNAP) is the principal enzyme of gene expression and regulation for all
three divisions of life: Eukaryota, Archaea and Bacteria. Recent progress in the structural and …

… Research in Sergei Borukhov's laboratory is funded by a grant from NIH. We are grateful
to reviewers of this manuscript for their comments and helpful suggestions. We apologize to …

X-ray structures of the universal translation initiation factor IF2/eIF5B have been determined
in three states: free enzyme, inactive IF2/eIF5B-GDP, and active IF2/eIF5B-GTP. IF2/eIF5B is …

… 48S complexes assemble correctly in rabbit reticulocyte lysate (RRL) 7 , which we therefore
used as a source from which to purify additional factor(s) required for assembly of the 48S …

… coli MRE600 were as described by Borukhov et al. (1992) with the exception that the
procedure was scaled up for 100 g of initial cell mass and that the dimensions of the phenyl-…

… 48S complexes were initially assembled on AUG triplets with eIF2, [ 35 S]Met- Formula (to
permit monitoring of its association), unlabeled GTP or 32 P-GTP (to permit monitoring of GTP …

We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase
(RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to …

The GreA and GreB transcript cleavage factors of Escherichia coli suppress elongation arrest
and may have a proofreading role in transcription. With the use of E. coli greA-greB- mutant…

… the very strong T7 A1 promoter located at a relatively long distance from the operator (S.Borukhov,
unpublished results). The difference in the promoter activity and its location relative to …