User profiles for S. M. Mangiameli

Sarah Mangiameli

MIT
Verified email at mit.edu
Cited by 475

In situ genome sequencing resolves DNA sequence and structure in intact biological samples

AC Payne, ZD Chiang, PL Reginato, SM Mangiameli… - Science, 2021 - science.org
INTRODUCTION Genomes are spatially organized across length scales from single base
pairs to whole chromosomes. This organization is thought to regulate gene expression and …

[PDF][PDF] Bifunctional immunity proteins protect bacteria against FtsZ-targeting ADP-ribosylating toxins

SY Ting, DE Bosch, SM Mangiameli, MC Radey… - Cell, 2018 - cell.com
ADP-ribosylation of proteins can profoundly impact their function and serves as an effective
mechanism by which bacterial toxins impair eukaryotic cell processes. Here, we report the …

Transcription leads to pervasive replisome instability in bacteria

SM Mangiameli, CN Merrikh, PA Wiggins, H Merrikh - Elife, 2017 - elifesciences.org
10.7554/eLife.19848.001 The canonical model of DNA replication describes a highly-processive
and largely continuous process by which the genome is duplicated. This continuous …

[HTML][HTML] The replisomes remain spatially proximal throughout the cell cycle in bacteria

SM Mangiameli, BT Veit, H Merrikh, PA Wiggins - PLoS Genetics, 2017 - journals.plos.org
The positioning of the DNA replication machinery (replisome) has been the subject of several
studies. Two conflicting models for replisome localization have been proposed: In the …

Photoselective sequencing: microscopically guided genomic measurements with subcellular resolution

SM Mangiameli, H Chen, AS Earl, JA Dobkin… - Nature …, 2023 - nature.com
In biological systems, spatial organization and function are interconnected. Here we present
photoselective sequencing, a new method for genomic and epigenomic profiling within …

The bacterial replisome has factory-like localization

SM Mangiameli, JA Cass, H Merrikh, PA Wiggins - Current genetics, 2018 - Springer
… et al. 2017) where we observed unexpected similarities between the organization in these
two highly divergent species (Mangiameli et al. 2017). The study, which used fluorescence …

The Structure and Dynamics of the Bacterial Replisome

SM Mangiameli - 2017 - digital.lib.washington.edu
Cellular reproduction relies on DNA replication. Many bacteria, including Bacillus subtilis
and Escherichia coli, possess only a single circular chromosome which is replicated bi-…

[PDF][PDF] Vizualizing Repliication Restart Process In Vivo with Single-Molecule Sensitivity

SM Mangiameli, PA Wiggins, H Merrikh, C Merrikh - Biophysical Journal, 2014 - cell.com
The rapid and faithful replication of the genome is of central importance to cell proliferation.
During this process, the replication machinery (replisome) frequently encounters obstacles, …

Lagging strand encoding promotes adaptive evolution

CN Merrikh, LA Harris, S Mangiameli, H Merrikh - bioRxiv, 2020 - biorxiv.org
Cells may be able to promote adaptive evolution in a gene-specific and temporally-controlled
manner. Genes encoded on the lagging strand have a higher mutation rate and evolve …

Research briefing

S Mangiameli, F Chen - nature.com
Photoselective sequencing is a new method for genomic and epigenomic profiling within
specific regions of a biological specimen that are chosen using light microscopy. This …