The recent boom in microfluidics and combinatorial indexing strategies, combined with low
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …

Recent technological advancements have enabled the profiling of a large number of genome-wide
features in individual cells. However, single-cell data present unique challenges that …

Importance More than 350 000 ventral hernias are repaired in the United States annually, of
which 75% are primary ventral hernias (eg, umbilical or epigastric hernias). Despite the …

Until recently, high-throughput gene expression technology, such as RNA-Sequencing (RNA-seq)
required hundreds of thousands of cells to produce reliable measurements. Recent …

We used the 10x Genomics Visium platform to define the spatial topography of gene expression
in the six-layered human dorsolateral prefrontal cortex. We identified extensive layer-…

Single-cell RNA-Seq (scRNA-Seq) profiles gene expression of individual cells. Recent
scRNA-Seq datasets have incorporated unique molecular identifiers (UMIs). Using negative …

Background The rapid development of single-cell RNA-sequencing (scRNA-seq) technologies
has led to the emergence of many methods for removing systematic technical noises, …

Background In high-throughput studies, hundreds to millions of hypotheses are typically
tested. Statistical methods that control the false discovery rate (FDR) have emerged as popular …

Background Current risk-assessment tools for surgical site occurrence (SSO) and surgical
site infection (SSI) are based on expert opinion or are not specific to open ventral hernia …

Single-cell gene expression technologies are powerful tools to study cell types in the human
brain, but efforts have largely focused on cortical brain regions. We therefore created a …