In April 2015, Public Health England implemented whole genome sequencing (WGS) as a
routine typing tool for public health surveillance of Salmonella, adopting a multilocus …

Background Shigellosis, traditionally a foodborne and waterborne infection, causes substantial
morbidity globally. It is now a leading cause of sexually transmitted gastroenteritis among …

Multilocus sequence typing (MLST) is an effective method to describe bacterial populations.
Conventionally, MLST involves Polymerase Chain Reaction (PCR) amplification of …

… Sequence type (ST) assignment was performed using a modified version of SRST, using
the MLST database described by Tewolde et al. (18). MOST software (for MLST) is available at …

Background. Mycobacterium abscessus has emerged as a major pathogen in cystic fibrosis
(CF) patients and has been associated with poor clinical outcomes, particularly following …

Sequence-based typing (SBT), analogous to multilocus sequence typing (MLST), is the
current “gold standard” typing method for investigation of legionellosis outbreaks caused by …

Following a large outbreak of foodborne gastrointestinal (GI) disease, a multiplex PCR
approach was used retrospectively to investigate faecal specimens from 88 of the 413 reported …

Advances in whole genome sequencing (WGS) platforms and DNA library preparation have
led to the development of methods for high throughput sequencing of bacterial genomes at …

Single-strain outbreaks of Streptococcus pyogenes infections are common and often go
undetected. In 2013, two clusters of invasive group A Streptococcus (iGAS) infection were …

Objectives To analyse genomic sequence data of referred Streptococcus pyogenes isolates
and those pertaining to selected elderly/nursing care or maternity clusters from 2010 to 2015 …