Deciphering the message in protein sequences: tolerance to amino acid substitutions
JU Bowie, JF Reidhaar-Olson, WA Lim, RT Sauer - Science, 1990 - science.org
An amino acid sequence encodes a message that determines the shape and function of a
protein. This message is highly degenerate in that many different sequences can code for …
protein. This message is highly degenerate in that many different sequences can code for …
[PDF][PDF] Proteomic discovery of cellular substrates of the ClpXP protease reveals five classes of ClpX-recognition signals
ClpXP is a protease involved in DNA damage repair, stationary-phase gene expression, and
ssrA-mediated protein quality control. To date, however, only a handful of ClpXP substrates …
ssrA-mediated protein quality control. To date, however, only a handful of ClpXP substrates …
[HTML][HTML] Sculpting the proteome with AAA+ proteases and disassembly machines
Abstract Machines of protein destruction—including energy-dependent proteases and
disassembly chaperones of the AAA+ ATPase family—function in all kingdoms of life to sculpt the …
disassembly chaperones of the AAA+ ATPase family—function in all kingdoms of life to sculpt the …
Transcription factors: structural families and principles of DNA recognition
CO Pabo, RT Sauer - Annual review of biochemistry, 1992 - annualreviews.org
… PABO & SAUER … PABO & SAUER … PABO & SAUER …
Protein-DNA recognition
CO Pabo, RT Sauer - Annual review of biochemistry, 1984 - annualreviews.org
… Sauer, manuscript in preparation). These include each of the five residues implicated in
specific base contacts (Tyr26 -+ Asp; Gln27 -+ His; Ser28 -+ Arg/ Asn; Lys32 -+ Thr/Gln; Arg38 -+ …
specific base contacts (Tyr26 -+ Asp; Gln27 -+ His; Ser28 -+ Arg/ Asn; Lys32 -+ Thr/Gln; Arg38 -+ …
AAA+ proteases: ATP-fueled machines of protein destruction
RT Sauer, TA Baker - Annual review of biochemistry, 2011 - annualreviews.org
… Moreover, ClpP dynamics have been proposed to open side pores transiently near the
ring-ring equatorial interface, which could provide a route for peptide egress (148). …
ring-ring equatorial interface, which could provide a route for peptide egress (148). …
Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA
KC Keiler, PRH Waller, RT Sauer - Science, 1996 - science.org
Variants of λ repressor and cytochrome b 562 translated from messenger RNAs without stop
codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and …
codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and …
The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system
…, E Roche, YN Zhou, RT Sauer - Genes & …, 1998 - genesdev.cshlp.org
Interruption of translation in Escherichia coli can lead to the addition of an 11-residue
carboxy-terminal peptide tail to the nascent chain. This modification is mediated by SsrA RNA (also …
carboxy-terminal peptide tail to the nascent chain. This modification is mediated by SsrA RNA (also …
[HTML][HTML] ClpXP, an ATP-powered unfolding and protein-degradation machine
TA Baker, RT Sauer - Biochimica et Biophysica Acta (BBA)-Molecular Cell …, 2012 - Elsevier
… In these complexes, the axial pores of the ClpX rings align with the pores in the ClpP rings,
providing a route into the proteolytic chamber. Consistently, electron density for translocating …
providing a route into the proteolytic chamber. Consistently, electron density for translocating …
[HTML][HTML] OMP peptide signals initiate the envelope-stress response by activating DegS protease via relief of inhibition mediated by its PDZ domain
NP Walsh, BM Alba, B Bose, CA Gross, RT Sauer - Cell, 2003 - cell.com
Transmembrane signaling between intracellular compartments is often controlled by regulated
proteolysis. Escherichia coli respond to misfolded or unfolded outer-membrane porins (…
proteolysis. Escherichia coli respond to misfolded or unfolded outer-membrane porins (…