Activating mutations in the receptor tyrosine kinase FLT3 are present in up to approximately
30% of acute myeloid leukemia (AML) patients, implicating FLT3 as a driver of the disease …

We present a CRISPR/Cas9 genome-editing strategy to systematically tag endogenous proteins
with fluorescent tags in human induced pluripotent stem cells (hiPSC). To date, we have …

Understanding how a subset of expressed genes dictates cellular phenotype is a considerable
challenge owing to the large numbers of molecules involved, their combinatorics and the …

Gene expression signatures are used in the clinic as prognostic tools to determine the risk
of individual patients with localized breast tumors developing distant metastasis. We lack a …

Treatment of AML patients with small molecule inhibitors of FLT3 kinase has been explored
as a viable therapy. However, these agents are found to be less than optimal for the …

… However, a small fraction of the γTuSC appears to oligomerize and migrate faster than the
10 S complex (Gunawardane, RN, and Y. Zheng, unpublished observation). This observation …

Publisher Summary This chapter discusses the purification and characterization of γ-tubulin
ring complex (γTuRC) and γ-tubulin small complex (γTuSC) and the function of γTuRC at the …

The Ras/RAF/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway plays
a central role in the regulation of cell growth, differentiation, and survival. Expression of …

To produce abundant cell culture samples to generate large, standardized image datasets
of human induced pluripotent stem (hiPS) cells, we developed an automated workflow on a …

Although some cell types may be defined anatomically or by physiological function, a
rigorous definition of cell state remains elusive. Here, we develop a quantitative, imaging-based …