Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle

…, J Zeman, S Wagner, P Beznosková… - Nucleic acids …, 2017 - academic.oup.com
Protein synthesis is mediated via numerous molecules including the ribosome, mRNA, tRNAs,
as well as translation initiation, elongation and release factors. Some of these factors play …

Short tRNA anticodon stem and mutant eRF1 allow stop codon reassignment

…, E Horáková, RL Ross, V Yurchenko, P Beznosková… - Nature, 2023 - nature.com
Cognate tRNAs deliver specific amino acids to translating ribosomes according to the
standard genetic code, and three codons with no cognate tRNAs serve as stop codons. Some …

Translation initiation factor eIF3 promotes programmed stop codon readthrough

P Beznosková, S Wagner, ME Jansen… - Nucleic acids …, 2015 - academic.oup.com
… (B) Quantification of selected factors from at least three independent experiments shown in
Figure 3A with corresponding P-values, where applicable. Western blot signals from each of …

Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast

P Beznosková, S Gunišová, LS Valášek - Rna, 2016 - rnajournal.cshlp.org
The molecular mechanism of stop codon recognition by the release factor eRF1 in complex
with eRF3 has been described in great detail; however, our understanding of what …

Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex

CE Aitken, P Beznosková, V Vlčkova, WL Chiu, F Zhou… - Elife, 2016 - elifesciences.org
10.7554/eLife.20934.001 Eukaryotic translation initiation factor 3 (eIF3) is a central player in
recruitment of the pre-initiation complex (PIC) to mRNA. We probed the effects on mRNA …

In-depth analysis of cis-determinants that either promote or inhibit reinitiation on GCN4 mRNA after translation of its four short uORFs

S Gunišová, P Beznosková, MP Mohammad, V Vlčková… - Rna, 2016 - rnajournal.cshlp.org
Translational control in eukaryotes is exerted by many means, one of which involves a
ribosome translating multiple cistrons per mRNA as in bacteria. It is called reinitiation (REI) and …

Increased expression of tryptophan and tyrosine tRNAs elevates stop codon readthrough of reporter systems in human cell lines

P Beznosková, L Bidou, O Namy… - Nucleic Acids …, 2021 - academic.oup.com
Regulation of translation via stop codon readthrough (SC-RT) expands not only tissue-specific
but also viral proteomes in humans and, therefore, represents an important subject of …

Structural integrity of the PCI domain of eIF3a/TIF32 is required for mRNA recruitment to the 43S pre-initiation complexes

…, T Kouba, P Neumann, P Beznosková… - Nucleic Acids …, 2014 - academic.oup.com
Transfer of genetic information from genes into proteins is mediated by messenger RNA (mRNA)
that must be first recruited to ribosomal pre-initiation complexes (PICs) by a mechanism …

Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough

P Beznosková, Z Pavlíková, J Zeman… - Nucleic Acids …, 2019 - academic.oup.com
Stop codon readthrough—the decoding of a stop codon by a near-cognate tRNA—is employed
by viruses to balance levels of enzymatic and structural proteins and by eukaryotic cells …

Cysteine tRNA acts as a stop codon readthrough-inducing tRNA in the human HEK293T cell line

LS Valášek, M Kučerová, J Zeman, P Beznosková - RNA, 2023 - rnajournal.cshlp.org
Under certain circumstances, any of the three termination codons can be read through by a
near-cognate tRNA; ie, a tRNA whose two out of three anticodon nucleotides base pair with …