Pseudomonas aeruginosa thrives in many aqueous environments and is an opportunistic
pathogen that can cause both acute and chronic infections. Environmental conditions and host …

The biosynthesis and export of bacterial cell-surface polysaccharides is known to occur
through several distinct mechanisms. Recent advances in the biochemistry and structural …

In mammals, S-adenosylhomocysteine hydrolase (AdoHcyase) is the only known enzyme to
catalyze the breakdown of S-adenosylhomocysteine (AdoHcy) to homocysteine and …

Extracellular polysaccharides comprise a major component of the biofilm matrix. Many
species that are adept at biofilm formation have the capacity to produce multiple types of …

Biofilm formation is a complex, ordered process. In the opportunistic pathogen Pseudomonas
aeruginosa, Psl and Pel exopolysaccharides and extracellular DNA (eDNA) serve as …

Allelic exchange is an efficient method of bacterial genome engineering. This protocol
describes the use of this technique to make gene knockouts and knock-ins, as well as single-…

… Because the enzymes function as adjuvants for the innate immune system and antibiotics,
they do not alter growth or metabolism, and therefore place no direct selective pressure on the …

Escherichia coli 5′-methylthioadenosine/S-adenosyl-homocysteine nucleosidase (MTAN)
hydrolyzes its substrates to form adenine and 5-methylthioribose (MTR) or S-…

High cellular concentrations of bis-(3′,5′)-cyclic dimeric guanosine mono-phosphate (c-di-GMP)
regulate a diverse range of phenotypes in bacteria including biofilm development. …

While complex intra- and interspecies microbial community dynamics are apparent during
chronic infections and likely alter patient health outcomes, our understanding of these …