Analyses of protein complexes are facilitated by methods that enable the generation of
recombinant complexes via coexpression of their subunits from multigene DNA constructs. …

The spatial organization, correct expression, repair, and segregation of eukaryotic genomes
depend on cohesin, ring‐shaped protein complexes that are thought to function by …

Sister chromatid cohesion depends on Sororin, a protein that stabilizes acetylated cohesin
complexes on DNA by antagonizing the cohesin release factor Wings-apart like protein (Wapl)…

Cohesin is a ring‐shaped protein complex that plays a crucial role in sister chromatid cohesion
and gene expression. The dynamic association of cohesin with chromatin is essential for …

Chromosome segregation depends on sister chromatid cohesion mediated by cohesin. The
cohesin subunits Smc1, Smc3, and Scc1 form tripartite rings that are thought to open at …

10.7554/eLife.21007.001 Stable kinetochore-microtubule attachment is essential for cell
division. It requires recruitment of outer kinetochore microtubule binders by centromere proteins …

Open (O) and closed (C) topologies of HORMA-domain proteins are respectively associated
with inactive and active states of fundamental cellular pathways. The HORMA protein O-…

Cyclin B: CDK 1 is the master kinase regulator of mitosis. We show here that, in addition to its
kinase functions, mammalian Cyclin B also scaffolds a localised signalling pathway to help …

10.7554/eLife.36764.001 Presence of multiple copies of the microtubule-binding NDC80
complex is an evolutionary conserved feature of kinetochores, points of attachment of …

The cohesin subunits Smc1, Smc3 and Scc1 form large tripartite rings which mediate sister
chromatid cohesion and chromatin structure. These are thought to entrap DNA with the help …