biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes
…, R VanderLinden, PJ Huis In't Veld… - Proceedings of the …, 2016 - National Acad Sciences
Analyses of protein complexes are facilitated by methods that enable the generation of
recombinant complexes via coexpression of their subunits from multigene DNA constructs. …
recombinant complexes via coexpression of their subunits from multigene DNA constructs. …
Rapid movement and transcriptional re‐localization of human cohesin on DNA
…, MP Zaczek, MI Molodtsov, PJ Huis in't Veld… - The EMBO …, 2016 - embopress.org
The spatial organization, correct expression, repair, and segregation of eukaryotic genomes
depend on cohesin, ring‐shaped protein complexes that are thought to function by …
depend on cohesin, ring‐shaped protein complexes that are thought to function by …
Aurora B and Cdk1 mediate Wapl activation and release of acetylated cohesin from chromosomes by phosphorylating Sororin
…, MM Sykora, PJ Huis in't Veld… - Proceedings of the …, 2013 - National Acad Sciences
Sister chromatid cohesion depends on Sororin, a protein that stabilizes acetylated cohesin
complexes on DNA by antagonizing the cohesin release factor Wings-apart like protein (Wapl)…
complexes on DNA by antagonizing the cohesin release factor Wings-apart like protein (Wapl)…
Cohesin acetylation and Wapl‐Pds5 oppositely regulate translocation of cohesin along DNA
M Kanke, E Tahara, PJ Huis in't Veld… - The EMBO journal, 2016 - embopress.org
Cohesin is a ring‐shaped protein complex that plays a crucial role in sister chromatid cohesion
and gene expression. The dynamic association of cohesin with chromatin is essential for …
and gene expression. The dynamic association of cohesin with chromatin is essential for …
Characterization of a DNA exit gate in the human cohesin ring
Chromosome segregation depends on sister chromatid cohesion mediated by cohesin. The
cohesin subunits Smc1, Smc3, and Scc1 form tripartite rings that are thought to open at …
cohesin subunits Smc1, Smc3, and Scc1 form tripartite rings that are thought to open at …
Molecular basis of outer kinetochore assembly on CENP-T
PJ Huis in't Veld, S Jeganathan, A Petrovic, P Singh… - Elife, 2016 - elifesciences.org
10.7554/eLife.21007.001 Stable kinetochore-microtubule attachment is essential for cell
division. It requires recruitment of outer kinetochore microtubule binders by centromere proteins …
division. It requires recruitment of outer kinetochore microtubule binders by centromere proteins …
CDC20 assists its catalytic incorporation in the mitotic checkpoint complex
Open (O) and closed (C) topologies of HORMA-domain proteins are respectively associated
with inactive and active states of fundamental cellular pathways. The HORMA protein O-…
with inactive and active states of fundamental cellular pathways. The HORMA protein O-…
Cyclin B1 scaffolds MAD 1 at the kinetochore corona to activate the mitotic checkpoint
…, M Camacho Reis, G Ciossani, PJ Huis in 't Veld… - The EMBO …, 2020 - embopress.org
Cyclin B: CDK 1 is the master kinase regulator of mitosis. We show here that, in addition to its
kinase functions, mammalian Cyclin B also scaffolds a localised signalling pathway to help …
kinase functions, mammalian Cyclin B also scaffolds a localised signalling pathway to help …
Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces
10.7554/eLife.36764.001 Presence of multiple copies of the microtubule-binding NDC80
complex is an evolutionary conserved feature of kinetochores, points of attachment of …
complex is an evolutionary conserved feature of kinetochores, points of attachment of …
[HTML][HTML] Topology and structure of an engineered human cohesin complex bound to Pds5B
MT Hons, PJ Huis in 't Veld, J Kaesler… - Nature …, 2016 - nature.com
The cohesin subunits Smc1, Smc3 and Scc1 form large tripartite rings which mediate sister
chromatid cohesion and chromatin structure. These are thought to entrap DNA with the help …
chromatid cohesion and chromatin structure. These are thought to entrap DNA with the help …