A unique transfer RNA (tRNA)/aminoacyl-tRNA synthetase pair has been generated that
expands the number of genetically encoded amino acids in Escherichia coli. When introduced …

… Plasmid DNA (100 ,ug) (54) was preincubated at 37C for 2 minutes with the above components,
after which suppressor tRNA (167 pg/ml of desalted and lyophilized tRNA suspended in …

Although chemists can synthesize virtually any small organic molecule, our ability to rationally
manipulate the structures of proteins is quite limited, despite their involvement in virtually …

Although it has long been recognized that the enteric community of bacteria that inhabit the
human distal intestinal track broadly impacts human health, the biochemical details that …

The development of new orthogonal aminoacyl-tRNA synthetase/tRNA pairs has led to the
addition of approximately 70 unnatural amino acids (UAAs) to the genetic codes of …

… stearothermophilus amber suppressor to selectively insert unnatural amino acids into
proteins in mammalian cells (PG Schultz, unpublished results), albeit currently in low yield. …

1 2 3 4 5 little is known about their mode of action on a molecular level.* 1 The study of
resistant strains has shown that a single missense mutation in the inhA gene of Mycobacterium …

The reversible phosphorylation of tyrosine residues is an important mechanism for
modulating biological processes such as cellular signaling, differentiation, and growth, and if …

The nervous system senses peripheral damage through nociceptive neurons that transmit a
pain signal 1 , 2 . TRPA1 is a member of the Transient Receptor Potential (TRP) family of ion …

Protein kinases have proved to be largely resistant to the design of highly specific inhibitors,
even with the aid of combinatorial chemistry 1 . The lack of these reagents has complicated …