In situ, reversible coacervate formation within lipid vesicles represents a key step in the
development of responsive synthetic cellular models. Herein, we exploit the pH responsiveness …

The ability to build synthetic cellular populations from the bottom-up provides the groundwork
to realize minimal living tissues comprising single cells which can communicate and …

… Error bars are taken from the standard deviation of the mean (n > 15). … d Plot showing the
vesicle stability over time with no decrease in the mean calcein fluorescence intensity (n > 10). …

In the field of bottom‐up synthetic biology, lipid vesicles provide an important role in the
construction of artificial cells. Giant unilamellar vesicles (GUVs), due to their membrane's …

The Q fever bacterium Coxiella burnetii replicates inside host cells within a large Coxiella-containing
vacuole (CCV) whose biogenesis relies on the Dot/Icm-dependent secretion of …

… showing the size distribution of MVVs and mean intensities of the inner LUVs (n = 123). …
where C enzymes/LUV is 1 μM for GOx and 1.7 μM for α-Glc and n LUV is given by the number of …

… (C and D) Binding isotherm curve obtained from co-sedimentation assays ((C), n = 3) and
QCM-D experiments ((D), n = 2) for the different proteins used in this study. Experimental …

HIV-1 Gag protein assembles at the plasma membrane of infected cells for viral particle
formation. Gag targets lipids, mainly PI(4,5)P2, at the inner leaflet of this membrane. Here, we …

For the past 2 decades, emerging single-nanopore technologies have opened the route to
multiple sensing applications. Besides DNA sensing, the identification of proteins and …

Biomimetic systems such as model lipid membranes are vital to many research fields including
synthetic biology, drug discovery and membrane biophysics. One of the most commonly …