Metagenomic sequencing is frequently claimed to have the potential to revolutionize microbiology
through rapid species identification and antimicrobial resistance (AMR) prediction. We …

We describe a gonorrhoea case with combined high-level azithromycin resistance and
ceftriaxone resistance. In February 2018, a heterosexual male was diagnosed with gonorrhoea in …

Double transgenic mice bearing fusion genes consisting of mouse albumin enhancer/promoter-mouse
c-myc complementary DNA and mouse metallothionein 1 promoter-human …

Illumina sequencing allows rapid, cheap and accurate whole genome bacterial analyses,
but short reads (<300 bp) do not usually enable complete genome assembly. Long-read …

Culture of multiple periprosthetic tissue samples is the current gold standard for microbiological
diagnosis of prosthetic joint infections (PJI). Additional diagnostic information may be …

Background Prosthetic joint infections are clinically difficult to diagnose and treat. Previously,
we demonstrated metagenomic sequencing on an Illumina MiSeq replicates the findings of …

Complete, accurate, cost-effective, and high-throughput reconstruction of bacterial genomes
for large-scale genomic epidemiological studies is currently only possible with hybrid …

Background Next-Generation Sequencing (NGS) is revolutionizing molecular epidemiology
by providing new approaches to undertake whole genome sequencing (WGS) in diagnostic …

10.7554/eLife.42486.001 Pyomyositis is a severe bacterial infection of skeletal muscle,
commonly affecting children in tropical regions, predominantly caused by Staphylococcus aureus…

Diagnosis of orthopedic device-related infection is challenging, and causative pathogens
may be difficult to culture. Metagenomic sequencing can diagnose infections without culture, …