Gate control: guard cell regulation by microbial stress

DH McLachlan, M Kopischke, S Robatzek - New Phytologist, 2014 - Wiley Online Library
Terrestrial plants rely on stomata, small pores in the leaf surface, for photosynthetic gas
exchange and transpiration of water. The stomata, formed by a pair of guard cells, dynamically …

Immobilized subpopulations of leaf epidermal mitochondria mediate PENETRATION2-dependent pathogen entry control in Arabidopsis

R Fuchs, M Kopischke, C Klapprodt, G Hause… - The Plant …, 2016 - academic.oup.com
The atypical myrosinase PENETRATION2 (PEN2) is required for broad-spectrum invasion
resistance to filamentous plant pathogens. Previous localization studies suggested PEN2-…

Chitin‐induced and CHITIN ELICITOR RECEPTOR KINASE1 (CERK1) phosphorylation‐dependent endocytosis of Arabidopsis thaliana LYSIN MOTIF‐CONTAINING …

J Erwig, H Ghareeb, M Kopischke, R Hacke… - New …, 2017 - Wiley Online Library
… Images for endosome quantification were taken using the Mark and find feature of the Leica
Las af software. For each imaging site, 12 consecutive focal plane images with a distance of …

The barley (Hordeum vulgare) cellulose synthase‐like D2 gene (HvCslD2) mediates penetration resistance to host‐adapted and nonhost isolates of the powdery …

…, MS Doblin, CT Beahan, M Kopischke… - New …, 2016 - Wiley Online Library
Cell walls and cellular turgor pressure shape and suspend the bodies of all vascular plants.
In response to attack by fungal and oomycete pathogens, which usually breach their host's …

[HTML][HTML] NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity

TJ Hawkins, M Kopischke, PJ Duckney… - Nature …, 2023 - nature.com
Members of the NETWORKED (NET) family are involved in actin-membrane interactions.
Here we show that two members of the NET family, NET4A and NET4B, are essential for …

A computational approach for inferring the cell wall properties that govern guard cell dynamics

HC Woolfenden, G Bourdais, M Kopischke… - The Plant …, 2017 - Wiley Online Library
… Four-week-old leaves at the same developmental stage were harvested in the morning after
a minimum of 3 h of light and transferred in a buffer [50 mm KCl, 10 μm CaCl 2 , 10 mm 2-(N-…

A novel A rabidopsis CHITIN ELICITOR RECEPTOR KINASE 1 (CERK1) mutant with enhanced pathogen‐induced cell death and altered receptor processing

…, M Stolze, U Lipka, M Kopischke… - New …, 2014 - Wiley Online Library
… For plasmolysis, leaf discs were mounted in 0.5 M NaCl before microscopy. … Full scans
within m/z of 300–1850 were recorded by the Orbitrap-FT analyzer at a resolution of 30 000 with …

Impaired sterol ester synthesis alters the response of Arabidopsis thaliana to Phytophthora infestans

M Kopischke, L Westphal, K Schneeberger… - The Plant …, 2013 - Wiley Online Library
… In a minimum of three experiments, three leaf disks of 9 mm in diameter of three different
plants per genotype were sampled and ground in 0.2 ml of 10 mm MgCl 2 . Bacterial growth …

[PDF][PDF] S-acylation stabilizes ligand-induced receptor kinase complex formation during plant pattern-triggered immune signaling

…, K Xhelilaj, S Myles, RL Pflughaupt, M Kopischke… - Current Biology, 2023 - cell.com
Plant receptor kinases are key transducers of extracellular stimuli, such as the presence of
beneficial or pathogenic microbes or secreted signaling molecules. Receptor kinases are …

Cell re-entry assays do not support models of pathogen-independent translocation of AvrM and AVR3a effectors into plant cells

B Petre, M Kopischke, A Evrard, S Robatzek… - BioRxiv, 2016 - biorxiv.org
… We thank M. Mbengue for discussions. BP was supported by an INRA Contrat Jeune
Scientifique (CJS), by the European Union, in the framework of the Marie-Curie FP7 COFUND …