RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing
technologies. Studies using this method have already altered our view of the extent …

The discovery of rare genetic variants is accelerating, and clear guidelines for distinguishing
disease-causing sequence variants from the many potentially functional variants present in …

Attempts to correlate protein abundance with mRNA expression levels have had variable
success. We review the results of these comparisons, focusing on yeast. In the process, we …

We report the generation and analysis of functional data from multiple, diverse experiments
performed on a targeted 1% of the human genome as part of the pilot phase of the ENCODE …

Determining the effect of gene deletion is a fundamental approach to understanding gene
function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype …

Eukaryotic cells make many types of primary and processed RNAs that are found either in
specific subcellular compartments or throughout the cells. A complete catalogue of these …

The GENCODE Consortium aims to identify all gene features in the human genome using a
combination of computational analysis, manual annotation, and experimental validation. …

Identification of protein–protein interactions often provides insight into protein function, and
many cellular processes are performed by stable protein complexes. We used tandem affinity …

The ENCyclopedia Of DNA Elements (ENCODE) Project aims to identify all functional elements
in the human genome sequence. The pilot phase of the Project is focused on a specified …

The accurate identification and description of the genes in the human and mouse genomes
is a fundamental requirement for high quality analysis of data informing both genome biology …