A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces
a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (…

GENETIC screening has identified a group ofmec (mechanosensory) genes that are required
for the function of a set of six touch-receptor neurons in the nematode Caenorhabditis …

… Martin Chalfie Martin Chalfie … We wish to thank Jane Mendel, WormBook Editor, for her
dedication to this collaboration, and Marty Chalfie for his vision. …

… (a result we also obtained; M. Chalfie and B. Davidson, unpublished data). Ward et ~ …
Finally, Chalfie et uLx found that the GFP fluorescence became stronger with illumination. This …

… Homer and M. Chalfie, unpublished data. There are only two lin-32 mutations. The more
severe of these affects all touch cells, but does not eliminate the anterior touch response ( 17). …

… Chalfie, unpublished data). A third possible explanation for the synaptic differences seen
among the touch cells is that synapse formation depends on when the cells are made. For …

… These mutations will be described in greater detail elsewhere (Chalfie, in preparation). …
of microtubules from only the microtubule cells (Chalfie, in preparation). The microtubule cells …

… For this work, we used the 0.5-m reflecting telescope and photoelectric photometer, following
a standardized procedure". Magnitudes determined on a dozen or so nights per season …

… between the apparent membrane potential at the stimulus site, V m ′, and the Nernst
potential for Na + ions. We estimated V m ′ by correcting V c for errors introduced by incomplete …

… In screens for touch insensitive mutants, eight EMS-induced and three insertional mutations
were identified (Chalfie and Sulston, 1981; M. Chalfie and M. Au, unpublished data). All …