User profiles for M. Akeson
Mark AkesonProfessor of Biomolecular Engineering, UC Santa Cruz Verified email at soe.ucsc.edu Cited by 16601 |
Three decades of nanopore sequencing
A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a
membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule …
membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule …
Nanopores and nucleic acids: prospects for ultrarapid sequencing
DNA and RNA molecules can be detected as they are driven through a nanopore by an
applied electric field at rates ranging from several hundred microseconds to a few milliseconds …
applied electric field at rates ranging from several hundred microseconds to a few milliseconds …
Nanopore sensors for nucleic acid analysis
… [51] Akeson M et al 1999 Microsecond time-scale discrimination among polycytidylic acid,
polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA …
polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA …
[HTML][HTML] The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community
… More recently, reads of more than 150 kb have routinely been achieved with the MinION (Akeson,
unpublished findings), and this is expected to improve in the next few months. …
unpublished findings), and this is expected to improve in the next few months. …
[PDF][PDF] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA …
… The reaction was stopped by adding 100 μl 3 M sodium acetate and 50 μl 10% glacial
acetic acid. The RNA was precipitated in ethanol, re-dissolved in TE buffer, then separated by …
acetic acid. The RNA was precipitated in ethanol, re-dissolved in TE buffer, then separated by …
Improved data analysis for the MinION nanopore sequencer
Speed, single-base sensitivity and long read lengths make nanopores a promising technology
for high-throughput sequencing. We evaluated and optimized the performance of the …
for high-throughput sequencing. We evaluated and optimized the performance of the …
[HTML][HTML] Nanopore sequencing and the Shasta toolkit enable efficient de novo assembly of eleven human genomes
De novo assembly of a human genome using nanopore long-read sequences has been
reported, but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable …
reported, but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable …
Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision
An emerging DNA sequencing technique uses protein or solid-state pores to analyze individual
strands as they are driven in single-file order past a nanoscale sensor 1 , 2 , 3 . However…
strands as they are driven in single-file order past a nanoscale sensor 1 , 2 , 3 . However…
Nanopore native RNA sequencing of a human poly (A) transcriptome
High-throughput complementary DNA sequencing technologies have advanced our
understanding of transcriptome complexity and regulation. However, these methods lose …
understanding of transcriptome complexity and regulation. However, these methods lose …
Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel
RNA and DNA strands produce ionic current signatures when driven through an α-hemolysin
channel by an applied voltage. Here we combine this nanopore detector with a support …
channel by an applied voltage. Here we combine this nanopore detector with a support …