… Whitfield, ML et al. Identification of genes periodically expressed in the human cell cycle …
Chang for critical reading of the manuscript, and members of the Whitfield laboratory for helpful …

Fibrosis is defined as an excessive deposition of connective tissue components and can
affect virtually every organ system, including the skin, lungs, liver and kidney. Fibrotic tissue …

Systemic sclerosis (SSc), also known as scleroderma, is a rare connective tissue disease
characterized by vascular and immune dysfunction, leading to fibrosis that can damage …

The genome-wide program of gene expression during the cell division cycle in a human
cancer cell line (HeLa) was characterized using cDNA microarrays. Transcripts of >850 genes …

… Ten million cells were treated with 200 ng/ml doxorubicin for 16 h, trypsinized and … We
added 1 ml of TRIzol to the sample, and RNA was extracted using the RNEasy Mini Kit (…

We used DNA microarrays representing >12,000 human genes to characterize gene
expression patterns in skin biopsies from individuals with a diagnosis of systemic sclerosis with …

Background Scleroderma is a clinically heterogeneous disease with a complex phenotype.
The disease is characterized by vascular dysfunction, tissue fibrosis, internal organ …

… or 12 ml of K-562 cells (starting density of ∼8 × 10 5 cells ml –1 … Adherent cells were washed
once in 10 ml of phosphate-… 12 ml of K-562 cells to a centrifuge tube, washed once in 10 ml …

… Ralph and Marian Falk Medical Research Trust grants to ML Whitfield. The nilotinib clinical
trial was also supported by an investigator-initiated grant from Novartis and by the Rudolph …

… were incubated in media with 5 ng/ml of human recombinant TGF2 (PeproTech) for up to 72
… ent cultures were incubated with TGF2 (5 ng/ml), rosiglitazone (10 M; Cayman Chemical), or …