[HTML][HTML] A rhesus macaque model of Asian-lineage Zika virus infection

…, J Post, N Schultz-Darken, ML Schotzko… - Nature …, 2016 - nature.com
… (c) Number of plaque-forming units per ml of serum for cohort 2 animals. (d) Viral RNA load
per ml of CSF collected on 4 and 14 dpi (e) Viral RNA load per vaginal swab collected on 0, 7…

[HTML][HTML] Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques

…, JM Hayes, N Schultz-Darken, ML Schotzko… - PLoS …, 2017 - journals.plos.org
… of 1.26 x 10 6 PFU/mL (equivalent to 1.43 x 10 9 vRNA copies/mL) was used for all 4
challenges. Animals were anesthetized as described above, and 1 mL of inocula was administered …

[HTML][HTML] Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection

…, JM Hayes, N Schultz-Darken, ML Schotzko… - PloS one, 2018 - journals.plos.org
mL of fluid was discarded due to potential maternal contamination, and an additional 3–4 mL
… The limit of detection is 33 copies/mL and the assay limit of quantification is 100 copies/mL, …

Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos

…, SG Eisele, MA Browne, ML Schotzko… - Proceedings of the …, 2001 - National Acad Sciences
Transgenic mice have provided invaluable information about gene function and regulation.
However, because of marked differences between rodents and primates, some areas of …

[HTML][HTML] Molecularly barcoded Zika virus libraries to probe in vivo evolutionary dynamics

…, E Peterson, W Newton, ML Schotzko… - PLoS …, 2018 - journals.plos.org
… , 100 U/ml penicillin, 100 µg/ml of streptomycin, and incubated … , 100 U/ml penicillin, 100
µg/ml of streptomycin, and incubated … ) per ml (equivalent to 2.01 x 10 10 vRNA copies per ml) of …

[HTML][HTML] Genome editing of CCR5 by CRISPR-Cas9 in Mauritian cynomolgus macaque embryos

…, MA Park, YH Kim, KD Mean, ML Schotzko… - Scientific reports, 2020 - nature.com
… Individual embryos were placed into a microwell of a CultureCoin MIRI-TL dish (Esco Medical,
Denmark) containing 25 μL of Global media overlaid with 3 mL of mineral oil. The medium …

Embryotoxic impact of Zika virus in a rhesus macaque in vitro implantation model

…, MT Aliota, TC Friedrich, ML Schotzko… - Biology of …, 2020 - academic.oup.com
… /mL bovine serum albumin (Sigma, Cat #A8806). A total of 40 million sperm were added to 2
mL of … with 0.1 mM sodium pyruvate and 0.1 mg/mL PVA. To facilitate capacitation, caffeine (…

Ferumoxytol dynamic contrast enhanced magnetic resonance imaging identifies altered placental cotyledon perfusion in rhesus macaques

…, R Chen, A Dhyani, A Zhu, ML Schotzko… - Biology of …, 2022 - academic.oup.com
… The total number of perfusion domains was calculated along with domain size (ml) and
blood flow rate (ml/min) for each placenta. Blood flow to each perfusion domain was calculated …

[HTML][HTML] Quantitative definition of neurobehavior, vision, hearing and brain volumes in macaques congenitally exposed to Zika virus

…, M Fritsch, M Dennis, LBC Teixeira, ML Schotzko… - PLoS …, 2020 - journals.plos.org
… The animals were anesthetized as described above, and 1 mL of inoculum at 1 x 10 4
PFU dilution in PBS was administered subcutaneously over the cranial dorsum. Post-inoculation, …

Pregnancy and live birth from nonsurgical transfer of in vivo‐ and in vitro ‐produced blastocysts in the rhesus monkey

…, L Knowles, MA Browne, ML Schotzko… - Journal of medical …, 2001 - Wiley Online Library
… The uterus was flushed dropwise into a collection bowl with 30 ml of dPBS, supplemented
with 1 mg/ml polyvinyl alcohol, and evaluated under a dissecting microscope to locate …