… Simonsen, Jonas Laurberg; Rollán, Cristina Hernández; Hoof, Jakob Blaesbjerg; Nørholm,
Morten Helge Hauberg; Batth, Tanveer Signh; Olsen, Jesper Velgaard; Johansen, Katja …

Proteins interacting with membranes via a single hydrophobic segment can be classified as
either monotopic or bitopic. Here, we probe the topology of a membrane-attached enzyme, …

Background The combined use of restriction enzymes with PCR has revolutionized molecular
cloning, but is inherently restricted by the content of the manipulated DNA sequences. …

Background We have developed a functional genomics approach based on expression cloning
in Xenopus oocytes to identify plant transporter function. We utilized the full-length cDNA …

BACterial Hosts for production of Bioactive phenolics from bERRY fruits (BacHBerry) was a
3-year project funded by the Seventh Framework Programme (FP7) of the European Union …

Membrane proteins are extremely challenging to produce in sufficient quantities for biochemical
and structural analysis and there is a growing demand for solutions to this problem. In …

… Daley, Morten HH Nørholm. A synbio approach for selection of highly expressed gene
variants in Gram-positive bacteria. Microbial Cell Factories 2018, 17 (1) https://doi.org/10.1186/…

… The Kastle–Meyer reagent is first prepared by reducing PHP with zinc dust, and it is then
applied to, for instance a textile swab with 0.1 MH 2 O 2 at pH 12. An immediate pink colour …

Interference with genes is the foundation of reverse genetics and is key to manipulation of
living cells for biomedical and biotechnological applications. However, classical genetic …

We report the biochemical characterization in Xenopus oocytes of the Arabidopsis thaliana
membrane protein, STP13, as a high affinity, hexose-specific H + -symporter. Studies with …