User profiles for M. C. Jewett

Michael C. Jewett

Stanford University
Verified email at stanford.edu
Cited by 16078

Cell-free gene expression: an expanded repertoire of applications

AD Silverman, AS Karim, MC Jewett - Nature Reviews Genetics, 2020 - nature.com
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

[HTML][HTML] Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

…, LE Yates, KJ Hsu, M Mrksich, MC Jewett… - Nature …, 2018 - nature.com
The emerging discipline of bacterial glycoengineering has made it possible to produce
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately, cell-…

[HTML][HTML] High-throughput preparation methods of crude extract for robust cell-free protein synthesis

YC Kwon, MC Jewett - Scientific reports, 2015 - nature.com
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization. …

Precise manipulation of chromosomes in vivo enables genome-wide codon replacement

…, NB Reppas, CJ Emig, D Bang, SJ Hwang, MC Jewett… - Science, 2011 - science.org
We present genome engineering technologies that are capable of fundamentally reengineering
genomes from the nucleotide to the megabase scale. We used multiplex automated …

[HTML][HTML] Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

…, FJ Isaacs, NL Kelleher, SH Hong, MC Jewett - Nature …, 2018 - nature.com
… To make cell extract, the thawed cells were suspended in 0.8 mL of S30 buffer per 1 g of
wet cell mass and processed as reported by Kwon and Jewett 59 . Full details for cell growth, …

Mimicking the Escherichia coli cytoplasmic environment activates long‐lived and efficient cell‐free protein synthesis

MC Jewett, JR Swartz - Biotechnology and bioengineering, 2004 - Wiley Online Library
… This is not possible with conventional secondary energy sources such as PEP, which
eventually poison the system (Jewett and Swartz, in press). Stability of pH is another attractive …

Cell-free protein synthesis: applications come of age

ED Carlson, R Gan, CE Hodgman, MC Jewett - Biotechnology advances, 2012 - Elsevier
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy
the growing demand for simple and efficient protein production. While used for decades as a …

How many human proteoforms are there?

…, CG Huber, AR Ivanov, ON Jensen, MC Jewett… - Nature chemical …, 2018 - nature.com
Despite decades of accumulated knowledge about proteins and their post-translational
modifications (PTMs), numerous questions remain regarding their molecular composition and …

Cell-free synthetic biology: thinking outside the cell

CE Hodgman, MC Jewett - Metabolic engineering, 2012 - Elsevier
Cell-free synthetic biology is emerging as a powerful approach aimed to understand, harness,
and expand the capabilities of natural biological systems without using intact cells. Cell-…

Cell‐free metabolic engineering: Biomanufacturing beyond the cell

QM Dudley, AS Karim, MC Jewett - Biotechnology journal, 2015 - Wiley Online Library
… For example, Jewett et al. demonstrated that formation of inverted lipid vesicles during extract
preparation facilitates oxidative phosphorylation which converts reducing equivalents from …