It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

The transcriptome is a powerful proxy for the physiological state of a cell, healthy or diseased.
As a result, transcriptome analysis has become a key tool in understanding the molecular …

In this review, we discuss transposon-insertion sequencing, variously known in the literature
as TraDIS, Tn-seq, INSeq, and HITS. By monitoring a large library of single transposon-…

The Rfam database (available via the website at http://rfam.sanger.ac.uk and through our
mirror at http://rfam.janelia.org ) is a collection of non-coding RNA families, primarily RNAs with …

Bacteria express many small RNAs for which the regulatory roles in pathogenesis have
remained poorly understood due to a paucity of robust phenotypes in standard virulence assays. …

The molecular roles of many RNA ‐binding proteins in bacterial post‐transcriptional gene
regulation are not well understood. Approaches combining in vivo UV crosslinking with RNA …

CRISPR-Cas systems recognize foreign genetic material using CRISPR RNAs (crRNAs). In
type II systems, a trans-activating crRNA (tracrRNA) hybridizes to crRNAs to drive their …

Many bacterial pathogens are specialized, infecting one or few hosts, and this is often
associated with more acute disease presentation. Specific genomes show markers of this …

The genus Yersinia has been used as a model system to study pathogen evolution. Using
whole-genome sequencing of all Yersinia species, we delineate the gene complement of the …

An epidemiological paradox surrounds Salmonella enterica serovar Enteritidis. In high-income
settings, it has been responsible for an epidemic of poultry-associated, self-limiting …