… The authors thank all the members of the Qi lab, as well as Dr. Fuguo Jiang and Arjun
Aditham for advice and helpful discussions. Thanks also to Matias Kaplan for preparing the …

CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled CRISPR …

The bacterial CRISPR–Cas9 system has emerged as a multifunctional platform for
sequence-specific regulation of gene expression. This Review describes the development of …

Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating,
perturbing, and engineering cellular systems. Here, we develop a method for controlling gene …

The genetic interrogation and reprogramming of cells requires methods for robust and
precise targeting of genes for expression or repression. The CRISPR-associated catalytically …

While the catalog of mammalian transcripts and their expression levels in different cell types
and disease states is rapidly expanding, our understanding of transcript function lags behind…

The spatiotemporal organization and dynamics of chromatin play critical roles in regulating
genome function. However, visualizing specific, endogenous genomic loci remains …

Signals in many biological processes can be amplified by recruiting multiple copies of
regulatory proteins to a site of action. Harnessing this principle, we have developed a protein …

Sequence-specific control of gene expression on a genome-wide scale is an important
approach for understanding gene functions and for engineering genetic regulatory systems. We …

Eukaryotic cells execute complex transcriptional programs in which specific loci throughout
the genome are regulated in distinct ways by targeted regulatory assemblies. We have …