ETS proteins are a group of evolutionarily related, DNA-binding transcriptional factors. These
proteins direct gene expression in diverse normal and disease states by binding to specific …

The use of isotopic substitution is a time-honoured method for simplifying the nuclear
magnetic resonance spectra of biological macromolecules. For example, the biosynthetic …

… that is characterized by (LP Mcintosh and TM Jovin, submitted… The ZB difference CD spectrum
(LP Mcintosh and TM Jovin, … families (30, 43; LP Mcintosh and TM Jovin, submitted). These …

Publisher Summary This chapter discusses the methods of biosynthetic enrichment of
proteins with 15 N in backbone and side-chain groups, as exemplified by the work with …

Using complementary approaches of potentiometry and NMR spectroscopy, we have determined
that the equilibrium acid dissociation constant (pK a value) of the arginine guanidinium …

… Hedberg, LA Plesniak, and LP McIntosh, unpublished data). The exchange broadening
of the δ- 13 C resonance of Glu172 near the midpoint of its titration may also result from this …

A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were
cloned, expressed and purified for structural studies. Of these,∼ 20% were found to be suitable …

Cell signaling that culminates in posttranslational modifications directs protein activity. Here
we report how multiple Ca 2+ -dependent phosphorylation sites within the transcription …

The pH optima of family 11 xylanases are well correlated with the nature of the residue
adjacent to the acid/base catalyst. In xylanases that function optimally under acidic conditions, …

The pK a values and charge states of ionizable residues in polypeptides and proteins are
frequently determined via NMR-monitored pH titrations. To aid the interpretation of the …