… of this strain were found to make dihydromonacolin L in vitro and to incorporate 13C from
13C-labeled malonyl-coenzyme A at the expected positions (K. Auclair & JC Vederas, unpubl.). …

The cytochrome P450 enzymes (P450s or CYPs) form a large family of heme proteins
involved in drug metabolism and in the biosynthesis of steroids, lipids, vitamins and natural …

Polyketides, the ubiquitous products of secondary metabolism in microorganisms, are made
by a process resembling fatty acid biosynthesis that allows the suppression of reduction or …

Metabolic regulation has been recognized as a powerful principle guiding immune
responses. Inflammatory macrophages undergo extensive metabolic rewiring 1 marked by the …

… The apparent K m for malonyl CoA is 0.5 mM and the V max is 1 × 10 - 7 M·min - 1 at an
enzyme concentration of 0.07 nM with the acetyl CoA concentration fixed at 0.60 mM in Tris-HCl …

Following activation, macrophages undergo extensive metabolic rewiring 1 , 2 . Production
of itaconate through the inducible enzyme IRG1 is a key hallmark of this process 3 . Itaconate …

… Karine Auclair. After graduating from the University of Ottawa in 2020 with an undergraduate
degree in biochemistry, she moved to the Auclair group to pursue an interest in green …

… (K i =14±4 μg/mL), and to a lesser extent, CYP1A2 (K i =106±24 μg/mL), CYP2E1 (… K i and
IC 50 values were calculated from nonlinear regressions using GraphPad Prism 4.0 and K i …

… K d values for CYP2E1 were measured by displacement of the pyrazole ligand and the … K
d was calculated using the following equation: K d = K s apparent /[1 + ligand concentration/K s …

Less than 9% of the plastic produced is recycled after use, contributing to the global plastic
pollution problem. While polyethylene terephthalate (PET) is one of the most common plastics…