Recent years have witnessed a sea change in our understanding of transcription regulation:
whereas traditional models focused solely on the events that brought RNA polymerase II (…

Precise spatio–temporal control of gene activity is essential for organismal development,
growth, and survival in a changing environment. Decisive steps in gene regulation involve the …

… In addition to P-TEFb, recent evidence demonstrates that a second Serine-2 CTD kinase,
comprised of Cyclin K and Cdk12 or Cdk13, also targets elongating Pol II, likely increasing …

Disclaimer: These recommendations are designed primarily as an educational resource for
medical geneticists and other healthcare providers to help them provide quality medical …

Clinicians are encouraged to document the reasons for the use of a particular procedure or
test, whether or not it is in conformance with this statement. Clinicians also are advised to …

… Genomic DNA was prepared using treatment with RNase A and proteinase K followed by
phenol-chloroform extraction. We carried out footprinting using ligation-mediated PCR …

It is widely assumed that the key rate-limiting step in gene activation is the recruitment of
RNA polymerase II (Pol II) to the core promoter 1 . Although there are well-documented …

Disclaimer: This statement is designed primarily as an educational resource for medical
geneticists and other clinicians to help them provide quality medical services. Adherence to this …

Metazoan transcription is controlled through either coordinated recruitment of transcription
machinery to the gene promoter or regulated pausing of RNA polymerase II (Pol II) in early …

Monomethylation of histone H3 at lysine 4 (H3K4me1) and acetylation of histone H3 at lysine
27 (H3K27ac) are correlated with transcriptionally engaged enhancer elements, but the …