Protein solubility is affected by ions. At low ion concentrations (<0.5 M), protein solubility
increases along with ionic strength. Ions in the solution shield protein molecules from the …

Structural coverage of the human kinome has been steadily increasing over time. The structures
provide valuable insights into the molecular basis of kinase function and also provide a …

Protein and lipid kinases play key regulatory roles in a number of biological processes.
Unsurprisingly, activating mutations in kinases have been linked to a number of disorders and …

The conformation of the pore region of the M2 proton channel depends on lipid bilayer
environment - Duong‐Ly - 2005 - Protein Science - Wiley Online Library … Duong-Ly …

Several metabolic enzymes undergo reversible polymerization into macromolecular
assemblies. The function of these assemblies is often unclear, but in some cases they regulate …

The protocol described here allows the student to construct a standard curve for a gel filtration
column with a separation range of 5–250 kD. The size (hydrodynamic radius) of a protein …

T cell-mediated adaptive immunity requires naïve, unstimulated T cells to transition from a
quiescent metabolic state into a highly proliferative state upon T cell receptor engagement. …

Small-molecule kinase inhibitors have typically been designed to inhibit wild-type kinases
rather than the mutant forms that frequently arise in diseases such as cancer. Mutations can …

Ion exchange chromatography (IEX) separates molecules by their surface charge, a property
that can vary vastly between different proteins. There are two types of IEX, cation exhange …

Expression of fusion proteins such as MBP fusions can be used as a way to improve the
solubility of the expressed protein in E. coli (Fox and Waugh, 2003; Nallamsetty et al., 2005; …