Mass spectrometry has played an integral role in the identification of proteins and their post-translational
modifications (PTM). However, analysis of some PTMs, such as phosphorylation…

The use of acidophilic, chemolithotrophic microorganisms capable of oxidizing iron and
sulfur in industrial processes to recover metals from minerals containing copper, gold and …

Figure 1. Sequence of events defining a contemporary mass spectrometry (MS)-based
proteomics experiment.(A) The process begins with a mixture of proteins from any number of …

Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem
mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene anions transfer an …

Mitogen‐activated protein kinase (MAP kinase) is a 42 kd serine/threonine protein kinase
whose enzymatic activity requires phosphorylation of both tyrosyl and threonyl residues. As a …

Protein kinases are coded by more than 2,000 genes and thus constitute the largest single
enzyme family in the human genome. Most cellular processes are in fact regulated by the …

Methodology for determining amino acid sequences of proteins by tandem mass spectrometry
is described. The approach involves enzymatic and/or chemical degradation of the protein …

Antigens recognized by T cells are expressed as peptides bound to major histocompatibility
complex (MHC) molecules. Microcapillary high-performance liquid chromatography-…

Of several thousand peptides presented by the major histocompatibility molecule HLA-A2.1,
at least nine are recognized by melanoma-specific cytotoxic T lymphocytes (CTLs). Tandem …

The functional significance of mono-, di-, and trimethylation of lysine residues within histone
proteins remains unclear. Antibodies developed to selectively recognize each of these …