CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled CRISPR …

Phenotypic drug discovery (PDD) approaches do not rely on knowledge of the identity of a
specific drug target or a hypothesis about its role in disease, in contrast to the target-based …

There has been a resurgence of interest in the use of phenotypic screens in drug discovery
as an alternative to target-focused approaches. Given that oncology is currently the most …

Biologists can now prepare and image thousands of samples per day using automation,
enabling chemical screens and functional genomics (for example, using RNA interference). …

To enable arrayed or pooled loss-of-function screens in a wide range of mammalian cell
types, including primary and nondividing cells, we are developing lentiviral short hairpin RNA (…

A new theory of gravity is proposed in which the geometry of space-time is determined by a
nonsymmetric field structure. The theory satisfies the following requirements:(1) general …

The mTOR kinase controls cell growth, proliferation, and survival through two distinct multiprotein
complexes, mTORC1 and mTORC2. mTOR and mLST8 are in both complexes, while …

Mutationally activated kinases define a clinically validated class of targets for cancer drug
therapy 1 . However, the efficacy of kinase inhibitors in patients whose tumours harbour such …

The ability to perturb genes in human cells is crucial for elucidating gene function and holds
great potential for finding therapeutic targets for diseases such as cancer. To extend the …

A covariant scalar–tensor–vector gravity theory is developed which allows the gravitational
constant G, a vector field coupling ω and the vector field mass μ to vary with space and time. …