In both normal and pathological states, cells respond rapidly to environmental cues by
synthesizing new proteins. The selective identification of a newly synthesized proteome has been …

Genome-wide association studies (GWAS) with intermediate phenotypes, like changes in
metabolite and protein levels, provide functional evidence to map disease associations and …

Recruitment of ubiquitinated proteins to the 26S proteasome lies at the heart of the ubiquitin-proteasome
system (UPS). Genetic studies suggest a role for the multiubiquitin chain …

As the speed with which proteomic labs generate data increases along with the scale of
projects they are undertaking, the resulting data storage and data processing problems will …

Reprogramming of somatic cells achieved by combination of the four transcription factors
Oct4, Sox2, Klf4, and c-Myc has very low efficiency. To increase the reprogramming efficiency …

p97 is an ATP-dependent chaperone that plays an important role in endoplasmic reticulum-associated
degradation but whose connections to turnover of soluble proteins remain sparse…

A major aim of proteomics is the identification of proteins in a given proteome at a given
metabolic state. This protocol describes the step-by-step labeling, purification and detection of …

Identifying genetic variants associated with circulating protein concentrations (protein
quantitative trait loci; pQTLs) and integrating them with variants from genome-wide association …

Embryonic stem (ES) cells are pluripotent cells isolated from mammalian preimplantation
embryos. They are capable of differentiating into all cell types and therefore hold great promise …

The mitochondrial division machinery regulates mitochondrial dynamics and consists of
Fis1p, Mdv1p, and Dnm1p. Mitochondrial division relies on the recruitment of the dynamin-…