… In some species, the Otto I buffer precipitates mucous substances, and nuclei released from
cells adhere to the precipitate (J. Doležel, unpubl. res.). On the other hand, nuclear isolation …

• Background Perusing the literature on nuclear ‘genome size’ shows that the term is not
stabilized, but applied with different meanings. It is used for the DNA content of the complete …

During the last fifteen years, flow cytometry and sorting greatly contributed to the improvement
of our knowledge of plant genome structure and function. This paper reviews the …

INTRODUCTION Wheat (Triticum aestivum L.) is the most widely cultivated crop on Earth,
contributing about a fifth of the total calories consumed by humans. Consequently, wheat …

An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum)
genome has been produced by sequencing isolated chromosome arms. We have annotated …

… Chopping should be done in a sandwich-like fashion rather than sequentially to make the
effects of cytosolic compounds as equable as possible (J. Loureiro, personal communication). …

Cereal grasses of the Triticeae tribe have been the major food source in temperate regions
since the dawn of agriculture. Their large genomes are characterized by a high content of …

… Kubaláková and J. Vrána for assistance with the preparation of DNA amplified from flow-…
J. Daron is funded by a grant from the French Ministry of Research. LP is funded by a grant …

Flow cytometric estimation of nuclear DNA content was performed in six plant species
employing three fluorochromes showing different DNA base preferences: propidium iodide (no …

We used a novel approach that incorporated chromosome sorting, next-generation
sequencing, array hybridization, and systematic exploitation of conserved synteny with model …