User profiles for J. E. Norville
Julie Erin NorvillePostdoctoral Scholar, Harvard Medical School Verified email at mit.edu Cited by 16431 |
RNA-guided human genome engineering via Cas9
Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly
interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems, that use …
interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems, that use …
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated
(Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide …
(Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide …
Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9
JF Li, JE Norville, J Aach, M McCormack, D Zhang… - Nature …, 2013 - nature.com
NATURE BIoTECHNoloGy volume 31 NumBeR 8 AuGuST 2013 689 was inserted to
minimize adverse effects on bacterial growth16 resulting from potential leaky expression and …
minimize adverse effects on bacterial growth16 resulting from potential leaky expression and …
Biocontainment of genetically modified organisms by synthetic protein design
…, MT Mee, R Takeuchi, G Kuznetsov, JE Norville… - Nature, 2015 - nature.com
Genetically modified organisms (GMOs) are increasingly deployed at large scales and in
open environments. Genetic biocontainment strategies are needed to prevent unintended …
open environments. Genetic biocontainment strategies are needed to prevent unintended …
Design, synthesis, and testing toward a 57-codon genome
…, G Tyree, A Gonzales, BL Wanner, JE Norville… - Science, 2016 - science.org
Recoding—the repurposing of genetic codons—is a powerful strategy for enhancing
genomes with functions not commonly found in nature. Here, we report computational design, …
genomes with functions not commonly found in nature. Here, we report computational design, …
7 Å projection map of the S-layer protein sbpA obtained with trehalose-embedded monolayer crystals
JE Norville, DF Kelly, TF Knight Jr, AM Belcher… - Journal of structural …, 2007 - Elsevier
Two-dimensional crystallization on lipid monolayers is a versatile tool to obtain structural
information of proteins by electron microscopy. An inherent problem with this approach is to …
information of proteins by electron microscopy. An inherent problem with this approach is to …
[HTML][HTML] Introduction of customized inserts for streamlined assembly and optimization of BioBrick synthetic genetic circuits
JE Norville, R Derda, S Gupta, KA Drinkwater… - Journal of biological …, 2010 - Springer
Background BioBrick standard biological parts are designed to make biological systems easier
to engineer (eg assemble, manipulate, and modify). There are over 5,000 parts available …
to engineer (eg assemble, manipulate, and modify). There are over 5,000 parts available …
Assembly of Radically Recoded E. coli Genome Segments
The large potential of radically recoded organisms (RROs) in medicine and industry depends
on improved technologies for efficient assembly and testing of recoded genomes for …
on improved technologies for efficient assembly and testing of recoded genomes for …
Fast and easy protocol for the purification of recombinant S‐layer protein for synthetic biology applications
JE Norville, DF Kelly, TF Knight… - Biotechnology …, 2011 - Wiley Online Library
A goal of synthetic biology is to make biological systems easier to engineer. One of the aims
is to design, with nanometer‐scale precision, biomaterials with well‐defined properties. The …
is to design, with nanometer‐scale precision, biomaterials with well‐defined properties. The …
A simple atomic force microscopy method for the visualization of polar and non-polar parts in thin organic films
A Amy Yu, PR Stone, JE Norville… - Journal of …, 2006 - Taylor & Francis
Here we present a scanning probe microscopy method that allows for the identification of
regions of different polarity (ie hydrophilicity) in thin organic films. This technique is based on …
regions of different polarity (ie hydrophilicity) in thin organic films. This technique is based on …