Listeria monocytogenes secretes two distinct phospholipases C, a phosphatidylinositol-specific
phospholipase C (PI-PLC) and a broad-range phospholipase C (PC-PLC). In this study, …

… (a) Light micrograph showing bacteria in pseudopod-like structures (arrowheads) at 8.5 h
postinfection. (b and c) Fluorescence micrographs of intracellular bacteria at 2 h postinfection. …

… This difference is also reflected in invasion of the liver 12 h later, suggesting that flagellum-mediated
motility enhances L. monocytogenes infectivity soon after bacterial ingestion in vivo. …

In monoderm (single‐membrane) Gram‐positive bacteria, the majority of secreted proteins
are first translocated across the cytoplasmic membrane into the inner wall zone. For a subset …

… (Marquis, H., unpublished observations). The observation that inhibition of acidification
decreases the efficiency of vacuolar lysis is consistent with the phenotype of bacteria deficient for …

… H: 250 cm x W: 300 cm x L: 300 cm) under a regulated temperature of 28 ± 2C, relative humidity
of 60–70%, and photoperiod of 12h ambient light and 12 h … in plastic containers (H: 8 cm …

The intracellular growth of several auxotrophic mutants of Listeria monocytogenes was
examined in cell culture, and virulence was evaluated in mice by intravenous injection of log-…

… The number of internalized bacteria per coverslip was determined at 1 h post-infection by
lysing infected cells in distilled water and plating appropriate serial dilutions of lysates onto LB (…

… in LB plus 8 mM MgSO4 and grown for 3 h at 37 C with shaking. The subculture was then …
on, the plates were incubated inverted for 18 h at 37 C. The plates were then photographed …

Listeriolysin O (LLO) is a pore-forming toxin that mediates phagosomal escape and cell-to-cell
spread of the intracellular pathogen Listeria monocytogenes. In order to identify factors …