All plants and green algae synthesize starch through the action of the same five classes of
elongation enzymes: the starch synthases. Arabidopsis mutants defective for the synthesis of …

Mutant lines defective for each of the four starch debranching enzyme (DBE) genes (AtISA1,
AtISA2, AtISA3, and AtPU1) detected in the nuclear genome of Arabidopsis (Arabidopsis …

A minimum of four soluble starch synthase families have been documented in all starch‐storing
green plants. These activities are involved in amylopectin synthesis and are extremely …

Chlamydomonas reinhardtii displays a diurnal rhythm of starch content that peaks in the
middle of the night phase if the algae are provided with acetate and CO 2 as a carbon source. …

… (F. Wattebled, unpublished data). The recent description of a mutant line of Arabidopsis
specifically defective for SS3 did not mention such a modification in the amylopectin structure (…

Among the three distinct starch phosphorylase activities detected in Chlamydomonas
reinhardtii, two distinct plastidial enzymes (PhoA and PhoB) are documented while a single …

This study assessed the impact on starch metabolism in Arabidopsis leaves of simultaneously
eliminating multiple soluble starch synthases (SS) from among SS1, SS2, and SS3. …

… E and F, Field emission type SEM images of dividing starch granule purified from … E and F,
Field emission type SEM images of dividing starch granule purified from synchronized cultures…

Genome editing is a powerful tool for plant functional genomics allowing for multiallelic
targeted mutagenesis. The recent development of Clustered Regularly Interspaced Short …

… The selection of homozygous mutant lines was performed by PCR amplification on genomic
DNA according to standard procedures described in Wattebled et al. (2008). RNA was …