Insulators are DNA sequence elements that can serve in some cases as barriers to protect a
gene against the encroachment of adjacent inactive condensed chromatin. Some insulators …

… Southern blot analysis of 24 and 48 h posttransfection plasmids demonstrated that
almost 50% of transiently transfected circular vectors are degraded (Recillas-Targa, unpublished …

… have suggested that CTCF functions as an insulator protein that can block the ability of
enhancers to activate promoters when placed between them in reporter assays (Recillas-Targa et …

… (A) DNA from immunoprecipitated (IP) or free (F) histone complexes was subjected to PCR
with primers specific to IL2R and globin sequences. The abundance of IL2R DNA detected in …

The 1.2-kb DNA sequence element (5′HS4) at the 5′ end of the chicken β-globin locus
has the two defining properties of an insulator: it prevents an “external” enhancer from acting …

… are required for protection against position effects (F.Recillas-Targa, unpublished). It has
been … If we define F target = moles of target sequence in IP acetylated fraction/moles of target …

The multifunctional CCCTC-binding factor (CTCF) protein exhibits a broad range of functions,
including that of insulator and higher-order chromatin organizer. We found that CTCF …

… (F) PEPC1 transcription is strongly increased in response to low Pi and is associated with
an upDAR. (G) NADP-MDH transcription is turned off in response to low Pi and is associated …

… De La Rosa-Velázquez I, Rincón-Arano H, BenítezBrbiesca L, Recillas-Targa F. Epigenetic
regulation of the human retinoblastoma tumor suppressor gene promoter by CTCF. Cancer …

Many genomic alterations associated with human diseases localize in noncoding regulatory
elements located far from the promoters they regulate, making it challenging to link …