Local adaptation: causal agents of selection and adaptive trait divergence
…, ML DeMarche, EB Josephs… - Annual review of …, 2022 - annualreviews.org
… ; (c) the agents of selection underlying local adaptation, when field experiments are paired
with environmental manipulations; (d) the processes that constrain local adaptation; and (e) …
with environmental manipulations; (d) the processes that constrain local adaptation; and (e) …
Genotype–environment associations to reveal the molecular basis of environmental adaptation
A fundamental goal in plant biology is to identify and understand the variation underlying
plants’ adaptation to their environment. Climate change has given new urgency to this goal, as …
plants’ adaptation to their environment. Climate change has given new urgency to this goal, as …
[HTML][HTML] The important contribution of transposable elements to phenotypic variation and evolution
NS Catlin, EB Josephs - Current opinion in plant biology, 2022 - Elsevier
Transposable elements (TEs) are responsible for significant genomic variation in plants.
Our understanding of the evolutionary forces shaping TE polymorphism has lagged behind …
Our understanding of the evolutionary forces shaping TE polymorphism has lagged behind …
Increasing the specificity of CRISPR systems with engineered RNA secondary structures
… e–h, Predicted structure of optimized hp-sgRNA spacers (hairpin 1 (e), hairpin 3 (f), hairpin
1 (g), hairpin 4 (h)); arrows indicate 3′ end of RNA. The sequences of the sgRNA variants …
1 (g), hairpin 4 (h)); arrows indicate 3′ end of RNA. The sequences of the sgRNA variants …
Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage
EA Josephs, DD Kocak, CJ Fitzgibbon… - Nucleic acids …, 2015 - academic.oup.com
CRISPR-associated endonuclease Cas9 cuts DNA at variable target sites designated by a
Cas9-bound RNA molecule. Cas9's ability to be directed by single ‘guide RNA’ molecules to …
Cas9-bound RNA molecule. Cas9's ability to be directed by single ‘guide RNA’ molecules to …
Hybrid origins and the earliest stages of diploidization in the highly successful recent polyploid Capsella bursa-pastoris
…, A Salcedo, K Holm, EB Josephs… - Proceedings of the …, 2015 - National Acad Sciences
… orientalis (4,000) but much smaller than the N e of the highly outcrossing C. grandiflora (…
that the N e of the founding population may be as large as 40,000. The reductions in N e in C. …
that the N e of the founding population may be as large as 40,000. The reductions in N e in C. …
[HTML][HTML] Co-Variation between Seed Dormancy, Growth Rate and Flowering Time Changes with Latitude in Arabidopsis thaliana
Life-history traits controlling the duration and timing of developmental phases in the life
cycle jointly determine fitness. Therefore, life-history traits studied in isolation provide an …
cycle jointly determine fitness. Therefore, life-history traits studied in isolation provide an …
[HTML][HTML] Evidence for Widespread Positive and Negative Selection in Coding and Conserved Noncoding Regions of Capsella grandiflora
The extent that both positive and negative selection vary across different portions of plant
genomes remains poorly understood. Here, we sequence whole genomes of 13 Capsella …
genomes remains poorly understood. Here, we sequence whole genomes of 13 Capsella …
What can genome‐wide association studies tell us about the evolutionary forces maintaining genetic variation for quantitative traits?
Understanding the evolutionary forces that shape genetic variation within species has long
been a goal of evolutionary biology. Integrating data for the genetic architecture of traits from …
been a goal of evolutionary biology. Integrating data for the genetic architecture of traits from …
Association mapping reveals the role of purifying selection in the maintenance of genomic variation in gene expression
EB Josephs, YW Lee… - Proceedings of the …, 2015 - National Acad Sciences
… As might be expected from its large effective population size (N e ) and relative lack of
population structure, purifying and positive selection are prevalent in C. grandiflora (4, 27), making …
population structure, purifying and positive selection are prevalent in C. grandiflora (4, 27), making …