… D through F, Transmission electron micrographs showing that the sarcomere ultrastructure
seen in wild-type animals (D) is largely preserved in heterozygous (E) and homozygous (F) …

… E) Direct oxygen-dependent changes in KDM activity and gene regulation [16]. HIF-independent
induction of KDMs is also possible. In all cases (C–E… and demethylation (Figure 2E). In …

BACKGROUND Animals and land plants are the most diverse complex multicellular life-forms
on Earth, and their success intricately links a capacity for adhering cells to perform …

… A PHD2 181–417 construct was expressed in E. coli and purified on a Ni-NTA column followed
by … PO Falnes, RF Johansen, E. Seeberg Nature 419, 178–182 (2002). … Emily Flashman …

… The N-terminally truncated form of PHD2 was chosen as it can be easily produced in E. coli.
… We assayed two other 2OG-dependent oxygenases; E. coli TauD and human PAHX, for …

… As further studies and mechanistic data emerge, it will be of interest to see if this stereoelectronic
effect is relevant to catalysis by other 2OG oxygenases. The structures will also be of …

… The N-terminal hexahistidine fusion was expressed in BL21-CodonPlus (DE3)-RIL E. coli
cells. The expression culture was induced with 1 mM isopropyl-β-D-thiogalactoside at optical …

… Interestingly, although GSK1278863 has the lowest observed E max , it is more active than
FG-4592 and Molidustat at low concentration ranges (Fig. 3A); the luciferase signals were …

… To test this, we produced recombinant human ADO and CDO1 in E. coli, reacted these
enzymes with synthetic peptides corresponding to residues 2 to 15 of human RGS4 and RGS5, …

… All available structures for these enzymes have a conserved double-stranded β-helix core
fold, which supports a highly, but not universally, conserved HXD/E…H triad of residues that is …