User profiles for D. Weitz

David A. Weitz

Professor of Physics and Applied Physics, Harvard University
Verified email at seas.harvard.edu
Cited by 161640

Droplet microfluidics for high-throughput biological assays

MT Guo, A Rotem, JA Heyman, DA Weitz - Lab on a Chip, 2012 - pubs.rsc.org
Droplet microfluidics offers significant advantages for performing high-throughput screens
and sensitive assays. Droplets allow sample volumes to be significantly reduced, leading to …

[HTML][HTML] Designer emulsions using microfluidics

…, JW Kim, A Fernandez-Nieves, CJ Martinez, DA Weitz - Materials Today, 2008 - Elsevier
We describe new developments for the controlled fabrication of monodisperse emulsions
using microfluidics. We use glass capillary devices to generate single, double, and higher …

The cell as a material

…, CP Brangwynne, GH Koenderink, DA Weitz - Current opinion in cell …, 2007 - Elsevier
To elucidate the dynamic and functional role of a cell within the tissue it belongs to, it is
essential to understand its material properties. The cell is a viscoelastic material with highly …

Monodisperse double emulsions generated from a microcapillary device

…, DR Link, PD Kaplan, HA Stone, DA Weitz - Science, 2005 - science.org
Double emulsions are highly structured fluids consisting of emulsion drops that contain
smaller droplets inside. Although double emulsions are potentially of commercial value, …

[PDF][PDF] Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets

…, N Kamitaki, EM Martersteck, JJ Trombetta, DA Weitz… - Cell, 2015 - cell.com
d Drop-seq enables highly parallel analysis of individual cells by RNA-seq d Drop-seq …
synthesis to generate the UMI on each oligo (Figure 1D); finally, an oligo-dT sequence (T30) is …

Colloidosomes: selectively permeable capsules composed of colloidal particles

…, MG Nikolaides, M Marquez, AR Bausch, DA Weitz - Science, 2002 - science.org
We present an approach to fabricate solid capsules with precise control of size, permeability,
mechanical strength, and compatibility. The capsules are fabricated by the self-assembly of …

[PDF][PDF] Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells

…, N Tallapragada, A Veres, V Li, L Peshkin, DA Weitz… - Cell, 2015 - cell.com
It has long been the dream of biologists to map gene expression at the single-cell level.
With such data one might track heterogeneous cell sub-populations, and infer regulatory …

Geometrically mediated breakup of drops in microfluidic devices

DR Link, SL Anna, DA Weitz, HA Stone - Physical review letters, 2004 - APS
… 2(d). An enlargement of the drop at its point of maximum extension " ‘e= we … out of the
junction in (d). At maximum extension, shown in (e), the extended length ‘e and width we give " …

Three-dimensional direct imaging of structural relaxation near the colloidal glass transition

…, JC Crocker, AC Levitt, A Schofield, DA Weitz - Science, 2000 - science.org
Confocal microscopy was used to directly observe three-dimensional dynamics of particles
in colloidal supercooled fluids and colloidal glasses. The fastest particles moved …

Single-cell analysis and sorting using droplet-based microfluidics

L Mazutis, J Gilbert, WL Ung, DA Weitz, AD Griffiths… - Nature protocols, 2013 - nature.com
We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …