… coli is unclear because tatE is expressed at a low level (71) and its deletion does not affect
the export of reporter fusions to any of the known Tat signal peptides (D. Tullman-Ercek, MP …

Gram-negative bacteria are attractive hosts for recombinant protein production because they
are fast growing, easy to manipulate, and genetically stable in large cultures. However, the …

One approach to reducing the costs of advanced biofuel production from cellulosic biomass
is to engineer a single microorganism to both digest plant biomass and produce …

As biological agents, viruses come in an astounding range of sizes, with varied shapes and
surface morphologies. The structures of viral capsids are generally assemblies of hundreds …

The type III secretion system (T3SS) exports proteins from the cytoplasm, through both the
inner and outer membranes, to the external environment. Here, a system is constructed to …

An Orbitrap-based ion analysis procedure determines the direct charge for numerous individual
protein ions to generate true mass spectra. This individual ion mass spectrometry (I 2 MS…

The Escherichia coli genome encodes at least 29 putative signal peptides containing a twin
arginine motif characteristic of proteins exported via the twin arginine translocation (Tat) …

The encapsulation of enzymes in nanometer-sized compartments has the potential to enhance
and control enzymatic activity, both in vivo and in vitro. Despite this potential, there is little …

… the CPEC library, Zachery Field for assisting with growth curve assays and site-directed
mutagenesis, Sahil Nallan for assisting with growth curve assays, and the entire Tullman-Ercek …

Introduction Cellulases are of great interest for application in biomass degradation, yet the
molecular details of the mode of action of glycoside hydrolases during degradation of …