User profiles for D. Siegal-Gaskins

Dan Siegal

Ginkgo Bioworks
Verified email at ginkgobioworks.com
Cited by 945

A photosensory two-component system regulates bacterial cell attachment

EB Purcell, D Siegal-Gaskins… - Proceedings of the …, 2007 - National Acad Sciences
… (D) ATPase activity of LovK is up-regulated by white light as measured by monitoring the …
the cells were induced with 500 μM isopropyl-β-d-thiogalactopyranoside (IPTG) for 4 h. Cells …

Gene circuit performance characterization and resource usage in a cell-free “breadboard”

D Siegal-Gaskins, ZA Tuza, J Kim… - ACS synthetic …, 2014 - ACS Publications
… At later times, the behavior of the system is no longer ideal and (d)/(dt)deGFP(t) and
MGapt(t) diverge, although there remain significant qualitative similarities between them (Supporting …

Interaction specificity, toxicity and regulation of a paralogous set of ParE/RelE‐family toxin–antitoxin systems

…, CM Castro Rojas, D SiegalGaskins… - Molecular …, 2010 - Wiley Online Library
D. Ribbon diagram of ParE 1 monomer structure (Dalton and Crosson, 2010). Sites of C-terminal
truncations are indicated by arrows and deleted portions of the sequence are shown in …

Minimally invasive determination of mRNA concentration in single living bacteria

…, L Bruneaux, TL Min, D Siegal-Gaskins… - Nucleic acids …, 2008 - academic.oup.com
… find D = 10 μm 2 /s. Using D = ω 2 /4ζ, where ω is the radius of the detection volume and ζ
the measured diffusion time of the beads (1.01 ms, Figure S4), we determined that ω = 0.2 μm. …

[PDF][PDF] Tightly regulated and heritable division control in single bacterial cells

D Siegal-Gaskins, S Crosson - Biophysical Journal, 2008 - cell.com
… (D) Simulations of diffusion under flow in the microfluidic culture channel show that small
molecules emitted from the cell do not accumulate in the channel. The concentration of a …

[HTML][HTML] Emergence of switch-like behavior in a large family of simple biochemical networks

D Siegal-Gaskins, MK Mejia-Guerra… - PLoS computational …, 2011 - journals.plos.org
Bistability plays a central role in the gene regulatory networks (GRNs) controlling many
essential biological functions, including cellular differentiation and cell cycle control. However, …

[HTML][HTML] The capacity for multistability in small gene regulatory networks

D Siegal-Gaskins, E Grotewold, GD Smith - BMC systems biology, 2009 - Springer
Background Recent years have seen a dramatic increase in the use of mathematical modeling
to gain insight into gene regulatory network behavior across many different organisms. In …

Biomolecular resource utilization in elementary cell-free gene circuits

D Siegal-Gaskins, V Noireaux… - 2013 American Control …, 2013 - ieeexplore.ieee.org
… We find that d[Y]/dt is completely insensitive to changes in RNAP concentration when
RNAP … On the other hand, the total concentration of ribosomes has a significant effect on d[Y]/dt, …

High‐resolution computational imaging of leaf hair patterning using polarized light microscopy

M Pomeranz, J Campbell, D SiegalGaskins… - The Plant …, 2013 - Wiley Online Library
The leaf hairs (trichomes) on the aerial surface of many plant species play important roles in
phytochemical production and herbivore protection, and have significant applications in the …

An analytical approach to bistable biological circuit discrimination using real algebraic geometry

D Siegal-Gaskins, E Franco… - Journal of The …, 2015 - royalsocietypublishing.org
Biomolecular circuits with two distinct and stable steady states have been identified as
essential components in a wide range of biological networks, with a variety of mechanisms and …