User profiles for D. N. Nesbeth

Darren N. Nesbeth

Associate Professor, Department of Biochemical Engineering, University College London
Verified email at ucl.ac.uk
Cited by 668

Synthetic biology routes to bio-artificial intelligence

DN Nesbeth, A Zaikin, Y Saka, MC Romano… - Essays in …, 2016 - portlandpress.com
… This scheme is proposed here by AZ and DN and the simulation was performed by CG and
… This scheme is proposed here by AZ and DN and the simulation was performed by CG and …

[HTML][HTML] Differential Endocytic Functions ofTrypanosoma brucei Rab5 Isoforms Reveal a Glycosylphosphatidylinositol-specific Endosomal Pathway

A Pal, BS Hall, DN Nesbeth, HI Field… - Journal of Biological …, 2002 - ASBMB
We demonstrate the presence of a glycosylphosphatidylinositol (GPI) anchor-specific
endosomal pathway in the protozoan pathogen Trypanosoma brucei. In higher eukaryotes …

Growth and productivity impacts of periplasmic nuclease expression in an Escherichia coli Fab' fragment production strain

DN Nesbeth, MA Perez‐Pardo, S Ali… - Biotechnology and …, 2012 - Wiley Online Library
Host cell engineering is becoming a realistic option in whole bioprocess strategies to maximize
product manufacturability. High molecular weight (MW) genomic DNA currently hinders …

Identification of black sturgeon caviar pigment as eumelanin

L Panzella, K Benning, DN Nesbeth, B Setaro… - Food Chemistry, 2022 - Elsevier
Reported herein is the purification of the pigment of black sturgeon caviar and its unambiguous
identification as a typical eumelanin by means of chemical degradation coupled with …

[PDF][PDF] Serum-free lentiviral vector production is compatible with medium-resident nuclease activity arising from adherent HEK293T host cells engineered with a …

…, J Ward, E Keshavarz-Moore, C Mason, DN Nesbeth - Heliyon, 2023 - cell.com
At present lentiviral vector production for cell and gene therapy commonly involves transient
plasmid transfection of mammalian cells cultivated in serum-containing media and addition …

Proteasome and thiol involvement in quality control of glycosylphosphatidylinositol anchor addition

B WILBOURN, DN Nesbeth… - Biochemical …, 1998 - portlandpress.com
Improperly processed secretory proteins are degraded by a hydrolytic system that is
associated with the endoplasmic reticulum (ER) and appears to involve re-export of lumenal …

Engineering an Autonucleolytic Mammalian Suspension Host Cell Line to Reduce DNA Impurity Levels in Serum-Free Lentiviral Process Streams

…, E Keshavarz-Moore, C Mason, DN Nesbeth - ACS Synthetic …, 2024 - ACS Publications
We engineered HEK293T cells with a transgene encoding tetracycline-inducible expression
of a Staphylococcus aureus nuclease incorporating a translocation signal. We adapted the …

Promoter engineering to optimize recombinant periplasmic Fab′ fragment production in Escherichia coli

…, A Templar, J Newton, DN Nesbeth - Biotechnology …, 2016 - Wiley Online Library
… The Nesbeth et al.25 procedure was used to assay total and extracellular dimeric Fab’ …
HPLC data gathered during this study and the studies of Balasundaram et al.28 and Nesbeth et al.…

Correction: Proteasome and thiol involvement in quality control of glycosylphosphatidylinositol anchor addition

B Wilbourn, DN Nesbeth, LJ Wainwright… - Biochemical …, 2024 - portlandpress.com
The authors of the original article “Proteasome and thiol involvement in quality control of
glycosylphosphatidylinositol anchor addition” DOI: 10.1042/bj3320111: Wilbourn et al., Biochem…

[HTML][HTML] Open source approaches to establishing Roseobacter clade bacteria as synthetic biology chassis for biogeoengineering

…, W Beaufoy, S Rose, T Ratisai, A Zaikin, DN Nesbeth - PeerJ, 2016 - peerj.com
… Cite this article Borg Y, Grigonyte AM, Boeing P, Wolfenden B, Smith P, Beaufoy W, Rose
S, Ratisai T, Zaikin A, Nesbeth DN. Open source approaches to establishing Roseobacter …