… (C) Transversal section of the central stem of the plant in A. (D-G) Same as A-C but for a
pmiR171-GFP transformant (sde1 background). (H-J) Same as A-C but for a GFP-171.1 …

… (C) The GFP sensors F1 to F11 were transfected together with (+) or in the absence of (–)
PFV-1. Their expression was assayed 48 hours later by Northern (first upper panel) and …

… The oven program was set as follows: 5 min at 40 C increasing to 320 C at 20 C/min, …
Interface temperature was fixed at 300 C to prevent re-condensation, ionization voltage was …

… (D and E) A leaf of a seedling from the plant in (C) imaged under UV without (D) or with (E) a
… (G–I) Silencing in a mature leaf of the plant in (C). (H) Acetic acid clearing of the leaf in (G). (I…

… at 4C, and centrifuged at 12,000 rpm for 5 min at 4C. The antibody directed against the HA
tag (Sigma) was coupled with protein A Sepharose, and immune complexes were centrifuged …

In the plant RNA interference (RNAi) pathway, 21-nucleotide duplexes of small interfering
RNA (siRNA) are processed from longer double-stranded RNA precursors by the RNaseIII …

… C for 10 min to activate hot start Taq DNA polymerase, followed by 42 cycles of denaturation
at 95 C for 15 s and annealing-extension at 62 C … steps of 1 C from 53–95 C to ensure that …

… cycling 45 times as follows: 95C for 10 s, 60C for 15 s and 72C for 30 s. The number of
cycles after which fluorescence reached a set threshold (C t value) was averaged for each …

… C for 2 min, then increase to 200 C at 15 C/min followed by a second increase to 300 C at 10
C… Program 2 was set as follows: 40 C for 2 min, then increase to 261 C at 13 C/min followed …

… Neither the NLS‐b nor the NLS‐c deletion caused a change in the nucleo‐cytoplasmic
distribution of GFP–P6ΔAΔ NLS (Figure 1B, panels 5 and 6). By contrast, its nuclear localization …